{"product_id":"immortalized-mouse-primary-brain-microvascular-endothelial-cells","title":"Immortalized Mouse Primary Brain Microvascular Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eImmortalized Mouse Primary Brain Microvascular Endothelial Cells from Cell Biologics are isolated from brain tissue of pathogen-free laboratory C57BL\/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. Immortalized Mouse Primary Brain Microvascular Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R\u0026amp;D System) or CD31\/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.\u003c\/p\u003e\n\u003cp\u003eImmortalized cell lines from Cell Biologics were derived from primary cells with extended life span. The primary cells were immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eM1168 Complete Endothelial Cell Medium w\/ Kit – 500 ml\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eCryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/C57-6023IM_C57-6023IM.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eC57-6023IM\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/C57-6023IM_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/C57-6023IM_1708462493.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003e1708462493\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/C57-6023IM_1698246847.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003e1698246847\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eInvestigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"0.5 x 10^6 cells","offer_id":52481744994485,"sku":"C57-6023IM","price":2800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/no_image-500x500_e83ddf20-eeab-4b55-bd39-e1c5c83dca85.webp?v=1778269063","url":"https:\/\/biofargo.com\/products\/immortalized-mouse-primary-brain-microvascular-endothelial-cells","provider":"Biofargo","version":"1.0","type":"link"}