{"product_id":"immortalized-human-sebaceous-gland-cells","title":"Immortalized Human Sebaceous Gland Cells","description":"\u003cstyle\u003e\n  .biofargo-product {\n    max-width: 1000px;\n    margin: 0 auto;\n    padding: 0;\n    color: #333333;\n    font-family: Arial, Helvetica, sans-serif;\n    font-size: 16px;\n    line-height: 1.7;\n  }\n\n  .biofargo-product h2 {\n    margin: 34px 0 14px;\n    padding: 0;\n    color: #333333;\n    font-size: 24px;\n    line-height: 1.3;\n    font-weight: 700;\n  }\n\n  .biofargo-product section:first-child h2 {\n    margin-top: 0;\n  }\n\n  .biofargo-product p {\n    margin: 0 0 14px;\n  }\n\n  .biofargo-product ul {\n    margin: 10px 0 0;\n    padding-left: 24px;\n  }\n\n  .biofargo-product li {\n    margin-bottom: 9px;\n  }\n\n  .biofargo-table-wrap {\n    display: block;\n    width: 100%;\n    max-width: 100%;\n    overflow-x: auto;\n    border: 1px solid #999999;\n    border-radius: 3px;\n    -webkit-overflow-scrolling: touch;\n  }\n\n  .biofargo-spec-table {\n    width: 100%;\n    min-width: 620px;\n    margin: 0;\n    border: 0;\n    border-collapse: collapse;\n    background: #ffffff;\n  }\n\n  .biofargo-spec-table th,\n  .biofargo-spec-table td {\n    padding: 10px 14px;\n    vertical-align: middle;\n    border-right: 1px solid #999999;\n    border-bottom: 1px solid #999999;\n  }\n\n  .biofargo-spec-table th:last-child,\n  .biofargo-spec-table td:last-child {\n    border-right: 0;\n  }\n\n  .biofargo-spec-table tbody tr:last-child td {\n    border-bottom: 0;\n  }\n\n  .biofargo-spec-table thead th {\n    color: #ffffff;\n    background: #0b5da9;\n    text-align: center;\n    font-weight: 700;\n  }\n\n  .biofargo-spec-table thead th:first-child,\n  .biofargo-spec-table tbody td:first-child {\n    width: 36%;\n  }\n\n  .biofargo-spec-table tbody td:first-child {\n    background: #f1f2f3;\n    text-align: center;\n    font-weight: 700;\n  }\n\n  .biofargo-spec-table tbody td:nth-child(2) {\n    background: #ffffff;\n    text-align: center;\n  }\n\n  .biofargo-faq {\n    width: 100%;\n    border-top: 1px solid #dddddd;\n  }\n\n  .biofargo-faq details {\n    margin: 0;\n    padding: 0;\n    border: 0;\n    border-bottom: 1px solid #dddddd;\n    border-radius: 0;\n    background: transparent;\n  }\n\n  .biofargo-faq summary {\n    padding: 12px 0;\n    color: #333333;\n    background: transparent;\n    cursor: pointer;\n    font-weight: 700;\n    line-height: 1.5;\n  }\n\n  .biofargo-faq details p {\n    margin: 0;\n    padding: 0 0 14px 18px;\n    color: #444444;\n  }\n\n  @media (max-width: 600px) {\n    .biofargo-product {\n      font-size: 15px;\n    }\n\n    .biofargo-product h2 {\n      font-size: 21px;\n    }\n\n    .biofargo-table-wrap {\n      overflow-x: auto;\n    }\n\n    .biofargo-spec-table {\n      min-width: 580px;\n    }\n\n    .biofargo-spec-table th,\n    .biofargo-spec-table td {\n      padding: 9px 12px;\n      font-size: 14px;\n    }\n\n    .biofargo-faq summary {\n      padding: 11px 0;\n    }\n  }\n\u003c\/style\u003e\n\u003carticle class=\"biofargo-product\"\u003e\n\u003csection id=\"description\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003ePrimary human sebocytes are difficult to obtain and lose proliferative capacity after a few passages, which limits their use in sebum, acne and skin barrier research. Biofargo Immortalized Human Sebaceous Gland Cells provide a stable, expandable human sebocyte model for in vitro studies.\u003c\/p\u003e\n\u003cp\u003eThe cells were derived from the sebaceous gland of a 30-year-old male donor and immortalized by lentiviral transduction of the SV40 large T antigen. They grow as adherent polygonal cells on gelatin-coated vessels in epithelial cell medium and can be subcultured at 1:3 to 1:5.\u003c\/p\u003e\n\u003c\/section\u003e\n\u003csection id=\"specification\"\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-table-wrap\" role=\"region\" tabindex=\"0\"\u003e\n\u003ctable class=\"biofargo-spec-table\"\u003e\n\u003cthead\u003e\u003ctr\u003e\n\u003cth scope=\"col\"\u003eItem\u003c\/th\u003e\n\u003cth scope=\"col\"\u003eSpecifications\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eCatalog Number\u003c\/td\u003e\n\u003ctd\u003eBFCL0142\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecies\u003c\/td\u003e\n\u003ctd\u003eHuman (Homo sapiens)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTissue Source\u003c\/td\u003e\n\u003ctd\u003eSebaceous gland\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDisease\u003c\/td\u003e\n\u003ctd\u003eUnknown\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDonor Age \/ Sex\u003c\/td\u003e\n\u003ctd\u003e30 years \/ Male\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMorphology\u003c\/td\u003e\n\u003ctd\u003ePolygonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eGrowth Properties\u003c\/td\u003e\n\u003ctd\u003eAdherent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eImmortalization\u003c\/td\u003e\n\u003ctd\u003eSV40 large T antigen, lentiviral transduction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCulture Medium\u003c\/td\u003e\n\u003ctd\u003eEpithelial cell complete medium\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eCulture Conditions\u003c\/td\u003e\n\u003ctd\u003e37 °C, 5% CO\u003csub\u003e2\u003c\/sub\u003e; 0.1% gelatin-coated vessels\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSubculture Ratio\u003c\/td\u003e\n\u003ctd\u003e1:3 to 1:5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMedium Change\u003c\/td\u003e\n\u003ctd\u003eHalf-medium change every other day\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBiosafety Level\u003c\/td\u003e\n\u003ctd\u003eBSL-1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection id=\"features\"\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eHuman sebocyte origin:\u003c\/strong\u003e Derived from human sebaceous gland tissue from a defined adult donor.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eExtended proliferation:\u003c\/strong\u003e SV40 large T antigen immortalization allows continuous expansion beyond the limited lifespan of primary sebocytes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAdherent epithelial growth:\u003c\/strong\u003e Polygonal adherent cells grown on gelatin-coated vessels with standard trypsin–EDTA passaging.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBSL-1 handling:\u003c\/strong\u003e Suitable for routine cell culture laboratories.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection id=\"application\"\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eSebum production and lipid metabolism studies.\u003c\/li\u003e\n\u003cli\u003eAcne and sebaceous gland disorder models.\u003c\/li\u003e\n\u003cli\u003eScreening of dermatological and cosmetic active ingredients.\u003c\/li\u003e\n\u003cli\u003eAndrogen, retinoid and hormone signaling in sebocytes.\u003c\/li\u003e\n\u003cli\u003eSkin inflammation and barrier function research.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection id=\"kit-components\"\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eImmortalized Human Sebaceous Gland Cells, 1 cryovial\u003c\/li\u003e\n\u003cli\u003eProduct datasheet\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection id=\"storage-shipping\"\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eShipped frozen.\u003c\/li\u003e\n\u003cli\u003eStore in liquid nitrogen. Storage at −80 °C reduces viability. Thaw and culture immediately upon receipt.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003csection id=\"faq\"\u003e\n\u003ch2\u003eFAQs\u003c\/h2\u003e\n\u003cdiv class=\"biofargo-faq\"\u003e\n\u003cdetails\u003e\u003csummary\u003eWhat medium should be used for these cells?\u003c\/summary\u003e\u003cp\u003eUse epithelial cell complete medium. Culture at 37 °C with 5% CO\u003csub\u003e2\u003c\/sub\u003e.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails\u003e\u003csummary\u003eDo the culture vessels need coating?\u003c\/summary\u003e\u003cp\u003eYes. Coat vessels with 0.1% gelatin at 37 °C for 1 h and wash once with PBS before seeding.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails\u003e\u003csummary\u003eHow were the cells immortalized?\u003c\/summary\u003e\u003cp\u003eThe cells were immortalized by lentiviral transduction of the SV40 large T antigen.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails\u003e\u003csummary\u003eHow should the cryovial be thawed?\u003c\/summary\u003e\u003cp\u003eThaw quickly in a 37 °C water bath for about 2 minutes, transfer to 5 mL complete medium, centrifuge at 200 × g for 5–10 min to remove the freezing medium, and seed into a gelatin-coated flask.\u003c\/p\u003e\u003c\/details\u003e\n\u003cdetails\u003e\u003csummary\u003eWhat subculture ratio is recommended?\u003c\/summary\u003e\u003cp\u003eSubculture at 1:3 to 1:5 by flask surface area using 0.25% trypsin–0.53 mM EDTA.\u003c\/p\u003e\u003c\/details\u003e\n\u003c\/div\u003e\n\u003c\/section\u003e\n\u003csection id=\"documents\"\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/BFCL0142_Immortalized_Human_Sebaceous_Gland_Cells_Datasheet_v2.pdf?v=1792463719\" target=\"_blank\" rel=\"noopener\"\u003eBFCL0142 Product Datasheet (PDF)\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/section\u003e\n\u003c\/article\u003e\n","brand":"Biofargo","offers":[{"title":"Frozen Vial","offer_id":53563038630069,"sku":"BFCL0142","price":1500.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/biofargo.com\/products\/immortalized-human-sebaceous-gland-cells","provider":"Biofargo","version":"1.0","type":"link"}