Description

Immortalized GFP-Expressing Rat Primary Small Intestinal Microvascular Endothelial Cells from Cell Biologics are isolated from the small intestinal tissue of Sprague-Dawley rats and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. Immortalized GFP-Expressing Rat Primary Small Intestinal Microvascular Endothelial Cells are characterized by use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

Immortalized cell lines from Cell Biologics are derived from primary cells expressing GFP. The primary cells are immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).

Suggested Medium

M1266 Complete Endothelial Cell Medium w/ Kit – 500 ml

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

RA-6054IM.GFP

Cell-Culture-Protocol-CB

1708462493

1698246847

Disclaimer

Investigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.