Description

Immortalized GFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells from Cell Biologics are isolated from bone marrow of pathogen-free laboratory C57BL/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen.

Immortalized GFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R&D System) or CD31/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

Immortalized GFP-Expressing Mouse Primary Bone Marrow-Derived Endothelial Cells can be used for cell-based adhesion, migration, vascular tube formation, transendothelial resistance (TER), western blot, immunoprecipitation, immunofluorescence flow cytometry, polymerase chain reaction, and generating cell derivatives for desired research applications.

Cell Biologics generated the immortalized cells by transducing human telomerase reverse transcriptase. The immortalized cells have an extended life span without altering the properties of the origin cells.

Suggested Medium

M1168 Complete Endothelial Cell Medium w/ Kit – 500 ml

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

C57-6221IM.GFP

Cell-Culture-Protocol-CB

1708462493

1698246847

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.