Description

Immortalized GFP-Expressing Monkey Primary Spleen Endothelial Cells from Cell Biologics are isolated from Cynomolgus Monkey spleen tissue and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. Immortalized GFP-Expressing Monkey Primary Spleen Endothelial are characterized by immunofluorescence staining with antibodies of CD31 (Catalog No. ab9498, Abcam), VE-cadherin (Catalog No. sc-28644, Santa Cruz, or Catalog No. ALX-210-232-C100, Enzo Life Sciences, Inc.), or use of fluorescence-labeled acetylated low-density lipoprotein (DiI-Ac-LDL) uptake (Catalog No. L-35353, Invitrogen), a functional marker for endothelial cells. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for more than 10 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

Immortalized cell lines from Cell Biologics are derived from primary cells expressing GFP. The primary cells are immortalized by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).

Suggested Medium

MK1168 Complete Monkey Endothelial Cell Medium – 125 ml

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

MK-6057IM.GFP

Cell-Culture-Protocol-CB

1708462493

1698246847

Disclaimer

Investigators should handle the cells with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at

http://www.cdc.gov/biosafety/publications/bmbl5/index.htm.

Immortalized GFP-Expressing Monkey Primary Spleen Microvascular Endothelial Cells