Description

Immortalized GFP-Expressing Canine Primary Colonic Fibroblasts from Cell Biologics are isolated from the colon tissue of beagle dog and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 2-3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. These cells are characterized by their spindle morphology and are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

Immortalized GFP-Expressing cells from Cell Biologics are derived from primary cells expressing GFP. The primary cells are Immortalized GFP-Expressing by over-expression of the large T-antigen of the simian virus (SV40) or human telomerase reverse transcriptase (hTERT).

Suggested Medium

M2267 Complete Fibroblast Medium w/ Kit (500 ml)

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

D-6231IM.GFP

Cell-Culture-Protocol-CB

1708462493

1698246847

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.