Bcakground

TGF-beta 3 (transforming growth factor beta 1) is one of three closely related mammalian members of the large TGF-beta superfamily that share a characteristic cystine knot structure . TGF-beta 1, -2 and -3 are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition . Each TGF-beta isoform has some non‑redundant functions; .Human TGF‑ beta 3 cDNA encodes a 412 amino acid (aa) precursor that contains a 23 aa signal peptide and a 389 aa proprotein . A furin‑like convertase processes the proprotein to generate an N‑terminal 277 aa latency‑associated peptide (LAP) and a C‑terminal 112 aa mature TGF‑ beta 3 (1-5).

Specifications

Synonyms	ARVD; ARVD1; FLJ16571; LDS5; RNHF; TGFB3; TGFbeta 3; TGF-beta 3; TGF-beta3; TGF-beta-3; transforming growth factor beta-3; transforming growth factor, beta 3.
Accession #	P10600.
Source	HEK293
Source	Ala301-Ser412
Predicted Moleucular weight	12.7 kDa (monomer)

Components and Storage

Formulation	Solution protein
	Dissolved in sterile 20 mM sodium citrate(pH=2.5) buffer .
	This solution can be diluted into other aqueous buers. Centrifuge the via prior to opening
Storage and Stability	Avoid repeated freeze-thaw cycles.
	It is recommended that the protein be aliquoted for optimal storage.
	12 months from date of receipt, -20 to -70 as supplied
Shipping	Shipping with dry ice

Quality

Purity	≥95%, determined by SDS-PAGE
Endotoxin Level	＜0.10 EU per 1 ug of the protein by the LAL method
Activity	Measured by the(CAGA) 12-luciferase reporter assay.
Activity	The EC50 for this effect is 0.29ng/mL

SDS-PAGE

Bioactivity

Documents

Human TGFb3