{"product_id":"human-primary-umbilical-vein-endothelial-cells-1","title":"Human Primary Umbilical Vein Endothelial Cells","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Umbilical Vein Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from human umbilical vein tissue. Human Primary Umbilical Vein Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10\u003csup\u003e6\u003c\/sup\u003e cells per ml. \u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eHuman Primary Umbilical Vein Endothelial Cells from \u003ci\u003eCell Biologics\u003c\/i\u003e display typical cobblestone with large dark nuclei appearance under light microscopy. Cells are tested for expression of endothelial cell marker using antibody, CD31 (Catalog No. 550389, BD; CD31\/PECAM-1 PE-conjugated Antibody, Catalog No. FAB3567P, R\u0026amp;D) or VE-Cadherin (FITC-VE-cadherin Catalog No. 560411, BD) by immunofluorescence staining or FACS. All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and\/or cell lots. Per request, a Certificate of Analysis will be provided for each cell lot purchased. Cells can be expanded for 3-5 passages under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003e Complete Human Endothelial Cell Medium– 500 ML\u003c\/p\u003e\n\u003cp\u003eCatalog No.  \u003cb\u003eH1168\u003c\/b\u003e\u003c\/p\u003e\n\u003ch2\u003eLaboratory Applications\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Umbilical Vein Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation. Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen\u003c\/p\u003e\n\u003cp\u003e(-180 °C) until ready for experimental use. Live cell shipment is also available on request.\u003c\/p\u003e\n\u003cp\u003eNever can primary cells be kept at -20 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/H-6207_H-6207.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6207\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/H-6207_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eAppropriate safety procedures should always be used with this material. Although Human Primary Umbilical Vein Endothelial Cells are isolated from normal human tissue, investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003ca href=\"http:\/\/www.cdc.gov\/biosafety\/publicati\"\u003ehttp:\/\/www.cdc.gov\/biosafety\/publicati\u003c\/a\u003eons\/bmbl5\/index.htm.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vail","offer_id":52481259929781,"sku":"H-6207","price":610.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":52481259962549,"sku":"H-6207-1","price":660.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/03072014_087_human_ECs-500x500_63e46cd7-0472-473d-9a89-9edd9b1adc82.jpg?v=1778252133","url":"https:\/\/biofargo.com\/products\/human-primary-umbilical-vein-endothelial-cells-1","provider":"Biofargo","version":"1.0","type":"link"}