{"product_id":"human-primary-tracheal-fibroblasts-1","title":"Human Primary Tracheal Fibroblasts","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Tracheal Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are isolated from normal human tracheal tissue. Human Primary Tracheal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003ci\u003eCell Biologics’\u003c\/i\u003e Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml.\u003c\/p\u003e\n\u003cp\u003e\u003cb\u003eProduct Testing\u003c\/b\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp\u003eHuman Primary Tracheal Fibroblasts from \u003ci\u003eCell Biologics\u003c\/i\u003e are tested for negative expression of von Willebrand Factor Expression\/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Human Primary Tracheal Fibroblasts are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003eFibroblast Medium \/w Kit –500 ml\u003c\/p\u003e\n\u003cp\u003eCatalog No.  \u003cb\u003eM2267\u003c\/b\u003e\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch2\u003eLaboratory Applications\u003c\/h2\u003e\n\u003cp\u003eHuman Primary Tracheal Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.\u003c\/p\u003e\n\u003cp\u003eNever can primary cells be kept at -20 °C.\u003cb\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/H-6217_H-6217.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eH-6217\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/H-6217_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eAppropriate safety procedures should always be used with this material. Although Human Primary Tracheal Fibroblasts are isolated from normal human tissue, investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all primary cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents. The entire text of discussing Biosafety in Microbiological and Biomedical Laboratories, 5th ed. is available online at \u003ca href=\"http:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm\"\u003ehttp:\/\/www.cdc.gov\/biosafety\/publications\/bmbl5\/index.htm\u003c\/a\u003e.\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vail","offer_id":52481257308341,"sku":"H-6217","price":690.0,"currency_code":"USD","in_stock":true},{"title":"T25 Flask","offer_id":52481257341109,"sku":"H-6217-1","price":740.0,"currency_code":"USD","in_stock":true},{"title":"T75 Flask","offer_id":52481257373877,"sku":"H-6217-2","price":980.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Fibroblasts-500x500_04a5dda3-95f6-4d2a-b101-a227ea2722fb.jpg?v=1778250028","url":"https:\/\/biofargo.com\/products\/human-primary-tracheal-fibroblasts-1","provider":"Biofargo","version":"1.0","type":"link"}