{"product_id":"human-nk-cell-purification-cocktail-k005","title":"Human NK Cell Purification Cocktail – K005","description":"\u003csection\u003e\n  \u003ch2\u003eDescription\u003c\/h2\u003e\n  \u003cp\u003eLaboratories isolating human NK cells from whole blood need to remove non-NK cells while keeping the target NK cells unlabeled for downstream experiments. The K005 Human NK Cell Purification Cocktail provides a negative-selection workflow for human whole blood. Its antibodies recognize specific surface markers on non-target cells and crosslink these cells to multiple red blood cells, forming dense immunorosettes.\u003c\/p\u003e\n  \u003cp\u003eDuring density gradient centrifugation, the immunorosettes sediment together with free red blood cells. The target human NK cells remain unlabeled and become highly enriched at the interface between the plasma and the density gradient medium, where they can be collected for subsequent laboratory use.\u003c\/p\u003e\n  \u003cp\u003eThis human NK cell purification cocktail is supplied as one kit in a 3 mL inner container measuring 16 mm × 35 mm. The product weight is 5 g. Before use, reconstitute the cocktail with 2 mL of sterile water. The recommended working amount is 40 µL of cocktail per 1 mL of human whole blood.\u003c\/p\u003e\n  \u003cp\u003e\u003cstrong\u003eImportant:\u003c\/strong\u003e Density gradient separation medium is not included with this product and must be supplied separately by the user.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eSpecification\u003c\/h2\u003e\n  \u003cdiv style=\"overflow-x:auto; -webkit-overflow-scrolling:touch;\"\u003e\n    \u003ctable style=\"width:100%; min-width:640px; border-collapse:collapse;\"\u003e\n      \u003ctbody\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eProduct Name\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eHuman NK Cell Purification Cocktail\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eCatalog Number\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eK005\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eSpecification\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e1 Kit\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eQuantity\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e1\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eInner Packaging Dimensions\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e16 mm × 35 mm\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eInner Container Size\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e3 mL\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eProduct Weight\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e5 g\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eSample Type\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eHuman whole blood\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eSelection Method\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eNegative selection using immunorosette formation and density gradient centrifugation\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eReconstitution\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eReconstitute with 2 mL of sterile water\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eRecommended Working Amount\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e40 µL per 1 mL of blood sample\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eRecommended Incubation\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e20 minutes at room temperature\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eRecommended Sample Condition\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eWhole blood collected within 24 hours and stored at 15–25°C\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eStorage\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003e−20°C\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eThawing Before Use\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eTransfer to 4°C in advance to thaw\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eDensity Gradient Separation Medium\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eNot included; must be supplied separately\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eTube Compatibility\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eUse standard centrifuge tubes; not compatible with lymphocyte separation tubes\u003c\/td\u003e\n        \u003c\/tr\u003e\n        \u003ctr\u003e\n          \u003cth scope=\"row\" style=\"text-align:left; padding:10px; border:1px solid #dddddd;\"\u003eIntended Use\u003c\/th\u003e\n          \u003ctd style=\"padding:10px; border:1px solid #dddddd;\"\u003eFor research use and further manufacturing only; not for direct use in humans\u003c\/td\u003e\n        \u003c\/tr\u003e\n      \u003c\/tbody\u003e\n    \u003c\/table\u003e\n  \u003c\/div\u003e\n  \u003cp\u003e\u003csmall\u003eThe 3 mL specification refers to the inner container size. The product is reconstituted with 2 mL of sterile water before use.\u003c\/small\u003e\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFeatures\u003c\/h2\u003e\n  \u003cul\u003e\n    \u003cli\u003eHuman NK cell purification from human whole blood.\u003c\/li\u003e\n    \u003cli\u003eNegative selection based on antibody-mediated immunorosette formation.\u003c\/li\u003e\n    \u003cli\u003eNon-target cells are crosslinked to multiple red blood cells.\u003c\/li\u003e\n    \u003cli\u003eImmunorosettes and free red blood cells sediment during density gradient centrifugation.\u003c\/li\u003e\n    \u003cli\u003eTarget NK cells remain unlabeled and are collected from the interface between the plasma and density gradient medium.\u003c\/li\u003e\n    \u003cli\u003eRecommended working amount of 40 µL per 1 mL of blood sample.\u003c\/li\u003e\n    \u003cli\u003eSupports workflows with or without the preparation of autologous inactivated plasma.\u003c\/li\u003e\n  \u003c\/ul\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eApplication\u003c\/h2\u003e\n  \u003cp\u003eThe K005 Human NK Cell Purification Cocktail is intended for the negative selection and enrichment of human NK cells from human whole blood. Following purification, the collected NK cells are suitable for the downstream applications specified below:\u003c\/p\u003e\n  \u003cul\u003e\n    \u003cli\u003eFlow cytometry\u003c\/li\u003e\n    \u003cli\u003eCell culture\u003c\/li\u003e\n    \u003cli\u003eDNA extraction\u003c\/li\u003e\n    \u003cli\u003eRNA extraction\u003c\/li\u003e\n  \u003c\/ul\u003e\n  \u003cp\u003eThis product is for research use and further manufacturing only and is not intended for direct use in humans.\u003c\/p\u003e\n\u003c\/section\u003e\n\n\u003csection\u003e\n  \u003ch2\u003eFAQ\u003c\/h2\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat is the K005 Human NK Cell Purification Cocktail used for?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eK005 is used to enrich human NK cells from human whole blood by negative selection. The purified cells can be used for flow cytometry, cell culture, or DNA\/RNA extraction.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow does the Human NK Cell Purification Cocktail work?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe antibodies in the cocktail recognize specific surface markers on non-NK cells and crosslink these cells to multiple red blood cells. The resulting dense immunorosettes sediment with free red blood cells during density gradient centrifugation, while the target NK cells remain unlabeled at the interface between the plasma and density gradient medium.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eCan K005 be used for NK cell isolation directly from human whole blood?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eYes. The documented sample type is human whole blood. For optimal performance, use whole blood collected within 24 hours and stored at room temperature between 15°C and 25°C.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow should K005 be reconstituted and used?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eReconstitute the Human NK Cell Purification Cocktail with 2 mL of sterile water. The recommended working amount is 40 µL of cocktail per 1 mL of blood sample, followed by incubation at room temperature for 20 minutes.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eIs density gradient separation medium included with K005?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eNo. Density gradient separation medium is not included with the product and must be supplied separately by the user.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat other materials are required for human NK cell purification?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe documented workflow requires human serum albumin at 20% concentration, Ficoll density gradient medium, DPBS, standard centrifuge tubes, pipettes, and other standard laboratory consumables. These materials are not identified as components of the K005 kit.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eCan lymphocyte separation tubes be used with this product?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eNo. K005 is not compatible with lymphocyte separation tubes. Standard centrifuge tubes must be used for blood separation.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eHow should the Human NK Cell Purification Cocktail be stored?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eUnused product should be stored at −20°C. Before use, transfer the product to 4°C in advance to thaw.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat centrifugation conditions are used for density gradient separation?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eAfter layering the diluted sample onto the density gradient medium, centrifuge at 1200 × g for 20 minutes using acceleration setting 1 and deceleration setting 0. Harvest the buffy coat layer and wash at 300 × g for 10 minutes using acceleration setting 8 and deceleration setting 6. Repeat the wash step once.\u003c\/p\u003e\n  \u003c\/details\u003e\n\n  \u003cdetails\u003e\n    \u003csummary\u003e\u003cstrong\u003eWhat blood volumes can be processed?\u003c\/strong\u003e\u003c\/summary\u003e\n    \u003cp\u003eThe workflow without preparation of autologous inactivated plasma specifies up to 15 mL of fresh whole blood per tube. The workflow that includes preparation of autologous inactivated plasma specifies up to 50 mL of fresh whole blood per tube.\u003c\/p\u003e\n  \u003c\/details\u003e\n\u003c\/section\u003e","brand":"EastMabBio","offers":[{"title":"Default Title","offer_id":53202706497717,"sku":"K005","price":199.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/exec-e8720feb-e02c-46c8-8fc9-fec3e8d0fd4b.png?v=1789109384","url":"https:\/\/biofargo.com\/products\/human-nk-cell-purification-cocktail-k005","provider":"Biofargo","version":"1.0","type":"link"}