Bcakground

Interleukin 23 is a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells.

Specifications

Synonyms	IL-23 p19/IL-12 p40; IL23; IL-23; IL-23p19/IL-12p40
Accession #	p40 (P29460) & p19 (AAG37232)
Source	Human embryonic kidney cell, HEK293-derived Human IL-23 proteir p40(Ile23-Ser328) & p19 (Arg20-Pro189).
Predicted Moleucular weight	36.5 kDa (p40) & 18.5 kDa (p19).

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer
	This solution can be diluted into other aqueous buffers. Centrifuge the via prior to opening.
Storage and Stability	Avoid repeated freeze-thaw cycles.
Storage and Stability	It is recommended that the protein be aliquoted for optimal storage. 12 months from date of receipt, -20 to -70 C as supplied.
Shipping	Shipping with dry ice.

Quality

Purity	>95%, determined by SDS-PAGE.
Endotoxin Level	<0.01 EU per 1 ug of the protein by the LAL method.
Activity	Measured by its ability to induce IL-17 secretion by mouse splenocytes. The EC50 for this effect is 0.25 pg/mL.

SDS-PAGE

Bioactivity

Documents

Human IL23