Bcakground

Interleukin-19 (IL-19) is a member of the IL-10 family of related cytokines. Its gene contains two alternate translation initiation sites, generating precursors of 215 amino acids (aa) and 177 aa, respectively. Both isoforms are processed to 17 kDa, 153 aa mature molecules. IL-19 contains seven helices and is secreted as a 35 kDa monomer. There are two potential N-linked glycosylation sites, and it is likely that the molecule is glycosylated. Mature human IL-19 shares 69% aa sequence identity with the mature mouse homologue. Although mouse IL-19 is active on human cells, human IL-19 is not active on mouse cells. IL-19 expression is limited to activated keratinocytes and monocytes. IL-19 binds a receptor complex consisting of the IL-20 receptor alpha (IL-20 R alpha, also known as IL-20 R1) and the IL-20 receptor beta (IL-20 R beta or IL-20 R2). This receptor complex is also shared by IL-20 and IL-24. Functionally, IL-19 induces IL-6 and TNF-alpha production by monocytes, and drives T-helper cell differentiation towards a Th2

Reference

1. Gallagher, G. et al. (2000) Genes Immun. 1:442.

2. Gallagher, G. et al. (2004) Int. Immunopharmacol. 4:615.

3. Laio, Y-C. et al. (2002) J. Immunol. 169:4288.

4. Romer, J. et al. (2003) J. Invest. Dermatol. 121:1306.

5. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.

Specifications

Synonyms	Human IL19; interleukin-19; IL19; IL-19; melanoma differentiation associated protein-like protein
Accession #	AAN40906
Source	Human embryonic kidney cell, HEK293-derived human IL-19 protein
Source	Leu25-Ala177
Predicted Moleucular weight	17.8 kDa

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer .
	This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Storage and Stability	Avoid repeated freeze-thaw cycles.
	It is recommended that the protein be aliquoted for optimal storage.
	12 months from date of receipt, -20 to -70 °C as supplied.
Shipping	Shipping with dry ice.

Quality

Purity	> 95%, determined by SDS-PAGE.
Endotoxin Stability	<0.010 EU per 1 ug of the protein by the LAL method.
Shipping	Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with human IL-20 R alpha and human IL-20 R beta.
Shipping	The EC50 for this effect is 0.2-1.0 ng/mL.

SDS-PAGE

Gel filtration

Bioactivity

Documents

Human IL19