Bcakground

Interon-alpha (IFN-alpha),also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction (1, 2). IFN-alpha has both anti-viral and immunomodulatory activities on target cells. Type I Interferons (IFNs) are well-known cytokines that exert antiviral activity, antitumor activity, and immunomodulatory effects. Interferon tau (IFNT), a type I IFN similar to alpha IFNs (IFNA), is the pregnancy recognition signal produced by the ruminant conceptus. Among the IFN-α genes, a total of 28 different sequence variants have been described. The three principal subtypes of IFNα-2 are designated α-2a, α-2b, and α-2c. IFNα-2b is being the predominant allele while IFNα-2a is less predominant and IFNα-2c only a minor allelic variant (2, 3).

Reference

1. Wang. et al. (2004) J Neuroimmunol. 156(1-2): 107-12.

2. Groopman JE, et al. (1984) Ann Intern Med. 100(5): 671-6.

3. Krueger JM, et al. (1987) Int J Immunopharmacol. 9(1): 23-30.

Specifications

Synonyms	Human IFNA2; IFNA2a; IFNalpha 2; IFN-alpha 2; IFN-alphaA; INFA2; interferon alpha A
Accession #	V00549
Source	Human embryonic kidney cell, HEK293-derived human IFN-alpha A protein
Source	Cys24-Glu188
Predicted Moleucular weight	19.2 kDa

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer
	This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Storage and Stability	Avoid repeated freeze-thaw cycles.
	It is recommended that the protein be aliquoted for optimal storage.
	12 months from date of receipt, -20 to -70 °C as supplied.
Shipping	Shipping with dry ice.

Quality

Purity	> 95%, determined by SDS-PAGE.
Endotoxin Level	<0.010 EU per 1 ug of the protein by the LAL method.
Activity	Measured in antiviral assays using WISH human amnion cells infected with vesicular stomatitis virus(VSV)
Activity	The EC50 for interferon in this assay is 0.5-3 pg/mL

SDS-PAGE

Gel filtration

Bioactivity

Documents

Human IFNA2