Bcakground

Epidermal growth factor (EGF) is a small, potent growth factor capable of inducing cell proliferation, differentiation, and survival. EGF is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin-binding EGF-like growth factor (HB-EGF), epigen, and the neuregulins (NRG)-1 through -6 (1). Members of The EGF family are characterized by a shared structural motif, the EGF-like domain, which contains three intramolecular disulfide bonds that are formed by six similarly spaced, conserved cysteine residues (2). These disulfide bonds are essential for proper protein conformation and receptor binding. All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The mature proteins are released from the cell surface by regulated proteolysis (1). The full length EGF protein is 1207 amino acids (aa) (EGF precursor) containing nine EGF domains and nine LDLR class B repeats. However, the mature protein is much smaller, only 53 aa, and is generated by proteolytic cleavage of the EGF domain proximal to the transmembrane region (3). EGF is well conserved across mammals with mature human EGF 70% identical to mature mouse and rat EGF. Physiologically, EGF is found in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid (4). EGF is a high affinity ligand of the EGF receptor (ErbB). Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members (5). EGF binding induces dimerization of the EGF receptor resulting in activation of the protein tyrosine kinase signaling pathway. These receptors undergo a complex pattern of ligand-induced homo- or hetero-dimerization to transduce EGF family signals (6, 7).

Reference

1. Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.

2. Carpenter, G. and Cohen, S. (1990) J. Biol. Chem. 265:7709.

3. Bell, G.I. et al. (1986) Nucl. Acids Res. 14:8427.

4. Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res. 164:1.

5. Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.

6. Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.

7. Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.

Specifications

Synonyms	beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEG
Accession #	P01133
Source	Human embryonic kidney cell, HEK293-derived human EGF protein
Source	Asn971-Arg1023
Predicted Moleucular weight	6.3 kDa

Components and Storage

Formulation	Solution protein
	Dissolved in sterile PBS buffer.
	This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
Storage and Stability	Avoid repeated freeze-thaw cycles.
	It is recommended that the protein be aliquoted for optimal storage.
	12 months from date of receipt, -20 to -70 °C as supplied.
Shipping	Shipping with dry ice

Quality

Purity	> 95%, determined by SDS-PAGE
Endotoxin Level	<0.010 EU per 1 ug of the protein by the LAL method
Activity	Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.
Activity	The ED50 for this effect is 4-50 pg/mL.

SDS-PAGE

Gel filtration

Bioactivity

Documents

Human EGF