Background

Adiponectin, also referred to as adipocyte complement-related protein of 30 kDa (Acrp30), is a protein secreted predominantly by adipocytes and exerts broad paracrine and endocrine effects on metabolic homeostasis and inflammation. It plays a critical role in promoting adipocyte differentiation, enhancing fatty acid oxidation, and improving insulin sensitivity. Circulating adiponectin levels are inversely associated with obesity, type 2 diabetes mellitus, and atherogenesis, highlighting its potential protective role in metabolic and cardiovascular disorders. Structurally, adiponectin oligomerizes into ~70 kDa noncovalently linked trimers, which are stabilized through disulfide bonds to form medium-molecular-weight hexamers and subsequently assemble into high-molecular-weight oligomers exceeding 300 kDa, the latter being considered the most biologically active isoforms.

Features

Species: Human
Size: 96 T
Sample Volume: 50 μl / 10 μl
Standard Curve Range: 6.86–5000 pg/ml
Assay Type: Fully Ready-to-Use
Sensitivity: 0.15 pg/ml (50 μl); 3.56 pg/ml (10 μl)
Operation Duration: 120 min
Assay Type: Sandwich ELISA

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0097	0.0102	0.0100
6.86	0.0277	0.0283	0.0280	0.0181
20.58	0.0665	0.0592	0.0629	0.0529
61.73	0.1897	0.1883	0.1890	0.1791
185.19	0.5615	0.5451	0.5533	0.5434
555.56	1.4160	1.4190	1.4175	1.4076
1666.67	2.8940	2.7930	2.8435	2.8336
5000.00	4.1026	4.1065	4.1046	4.0946

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	103.3	485.3	1632.6	111.5	520.8	1724.6
Standard Deviation	2.8	27.8	81.6	5.8	31.7	128.9
Coefficient of Variation(%)	2.7	5.7	5.0	5.2	6.1	7.5

Intra-assay Precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (precision between assays): Three samples of known concentration were tested six times on different plates to assess inter-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking three levels of human adiponectin into healthy human serum samples. The unspiked serum was used as a blank in this experiment.

The recovery ranged from 81% to 126%, with an overall mean recovery of 93%.

Sample values

Sample Matrix

Sample Evaluated

Range (ng/ml)

Detectable

%

Mean of Detectable (ng/ml)

Serum

30

2621.9-7072.8

100

4559.1

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human adiponectin in this assay. No medical histories were available for the donors.

Storage

Store at +4 °C. Please refer to the protocols.

Research Use Only

For research use only. Not for use in diagnostic procedures.

Documents

Human Adiponectin ELISA Kit