Description

This product is a chemically modified Taq DNA polymerase for use with HotStart PCR, which inhibits the activity of Taq enzyme due to its chemical modification. Taq DNA polymerase activity was completely inhibited at room temperature, but inhibition failed at 95°C for 10 minutes, Taq enzyme activity was restored, and DNA could be polymerized, which inhibited non-specific amplification due to non-specific primer annealing or primer dimers at low temperatures. In addition, the product did not detect 3' to 5' school exonuclease activity and can be used for qPCR detection.

Specification

Parameter	Specification
Package	1 KU/200 U/500 U
Full name	HotStart Taq DNA Polymerase（chemical mod
Composition	HotStart Taq DNA polymerase (5U/ul), 5X PCR buffer (containing 10 mM MgCl2).
Reaction Buffer	10X PCR Buffer
Unit Definition	The amount of enzyme required to precipitate 10 nmol of dNTPs into acid-insoluble precipitate at 75°C for 30 min was defined as 1 activity unit (U).
Concentration	5 U/μl
Component	HotStart Taq DNA polymerase (5U/ul), 5X PCR buffer (containing 10 mM MgCl2).

Applications

PCR amplification of complex template DNA, PCR amplification of complex template cDNA (RT-PCR), sequencing of single- or double-stranded DNA, site-directed mutagenesis, real-time PCR.

Storage

-20°C

HotStart Taq DNA Polymerase(chemical mod