{"product_id":"hot-start-ttx-dna-kit","title":"Hot Start TTx (DNA) Kit-Toyobo","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eHot Start TTx (DNA) Kit is qPCR reagent based on our original polymerase, TTx DNA Polymerase. TTx DNA Polymerase has higher amplification efficiency than Taq DNA Polymerase, which is a general-purpose enzyme, and enables amplification using fast cycle condition and amplification from a crude sample containing PCR inhibitors.\u003c\/p\u003e\n\u003cp style=\"margin-top: 0;\"\u003eIn addition, TTx DNA Polymerase has a 5 '- 3' exonuclease activity, so it can be used for real-time PCR using probe assays such as TaqMan ® assay. This enzyme contains neutralizing antibodies, thus allowing for Hot start PCR.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eExcellent DNA Amplification Efficiency\u003c\/strong\u003e\u003cbr\u003eThe reaction composition is optimized based on TTx DNA Polymerase. TTx DNA Polymerase has higher elongation capacity than general-purpose enzymes such as Taq DNA Polymerase and Tth DNA Polymerase.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eDetectability with high-speed cycles\u003c\/strong\u003e\u003cbr\u003eTaking advantage of the high amplification efficiency, TTx DNA Polymerase allows efficient amplification even with high-speed cycles.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eTolerant of PCR Inhibitors\u003c\/strong\u003e\u003cbr\u003eThis kit is effective for amplification from crude samples (e.g., biological samples,\u003cbr\u003efoodstuffs, soil extract, etc.). In the case of amplification from whole blood, sufficient\u003cbr\u003eamplification can be achieved by adding it directly to the reaction solution without purification of nucleic acid.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eAllows multiplex PCR\u003c\/strong\u003e\u003cbr\u003eBy using TaqMan® probes with different detection wavelengths, it is possible to detect more than one target simultaneously. You can detect control genes and target genes within the same reaction, allowing rapid, simple, and accurate gene quantification.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e \u003cstrong\u003eUtilization of dUTP\u003c\/strong\u003e\u003cbr\u003eThis kit contains dUTP instead of dTTP in 2x Buffer for rTth\/ TTx (DNA). Therefore, the rate of false-positive detection can be reduced by adding Uracil-N-glycosylase (UNG).\u003cbr\u003e*UNG is not supplied with this kit.Uracil-DNA Glycosylase (UNG), Heat-labile(Code No. UNG-101) can be used.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication\u003c\/h2\u003e\n\u003cp style=\"padding-left: 5px;\"\u003eTaqMan® assays or hybridization probe assays using DNA template\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eStorage condition\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eStore at -20℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eComponents\u003c\/h2\u003e\n\u003cp\u003eThis kit includes the following components for 250 reactions, 20 μL total reaction volume. All reagents should be stored at -20°C.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eHSTTX-101\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e2x Buffer for rTth\/ TTx (DNA)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1.25 mL x 2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eHot Start TTx DNA Polymerase (4U\/ μL)\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e62.5 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e2x Reaction Buffer contains essential components for the reaction (buffer, salts, Mg2+, dATP, dCTP, dGTP, and dUTP, etc.). Add template DNA, primers, and attached Hot Start TTx DNA Polymerase, and adjust to 1x concentration with sterile water etc.\u003c\/p\u003e\n\u003cp\u003eDNA Polymerase is a mixture of TTx DNA polymerase and hot start antibodies. Its concentration is 4U\/ μL.\u003c\/p\u003e\n\u003cp\u003eThis kit doesn’t contain a passive reference dye (ROX). When using a passive reference dye to compensate fluorescence intensity and dispensing error between wells, please use the separately sold 50x ROX reference dye (Code No. ROX-101).\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication Data\u003c\/h2\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 1.Detection of Bordetella pertussis IS481 gene and human G6PDH gene.\u003c\/h3\u003e\n\u003cp\u003eAmplifications of the IS481 gene using B. pertussis DNA (150,000, 3,000, 600, 120, 24, 5, and 1 copies) or the G6PDH gene using 5-fold dilutions (four steps) of cDNA from HeLa cell total RNA were performed using real-time PCR reagents (Hot Start TTx or Taq DNA Polymerase). We obtained efficient amplification of all targets using the Hot Start TTx kit.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-012_01.png?v=1775530752\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 2.Real-time PCR with high-speed cycles\u003c\/h3\u003e\n\u003cp\u003eAmplifications of African swine fever viral DNA were performed using real-time PCR reagents (Hot Start TTx or Taq DNA Polymerase) with high-speed cycles. Only the Hot Start TTx (DNA) Kit showed efficient amplification in fast-cycling conditions.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-012_02.png?v=1775530752\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 3.Effect of adding blood plasma to reactions\u003c\/h3\u003e\n\u003cp\u003eAmplifications of African swine fever viral DNA were performed using real-time PCR reagents (Hot Start TTx or Taq DNA Polymerase). Plasma (2.5 µL) was included in the 20µL reaction solution. The Taq DNA Polymerase-based reagents were inhibited by the plasma and no amplification was observed. However, amplification occurred with TTx DNA Polymerase, without inhibition.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-012_03.png?v=1775530752\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 4.Detection of enterobacteria by multiplex PCR\u003c\/h3\u003e\n\u003cp\u003eAmplifications of four target genes* in one reaction were performed using real-time PCR reagents (Hot Start TTx or Taq DNA Polymerase). The target genes were difficult to detect with the Taq DNA Polymerase-based reagents, whereas with the Hot Start TTx (DNA) Kit, all four targets were detectable.\u003cbr\u003e*The target genes were derived from Salmonella, Shigella and Escherichia coli O157 plus an internal control(IC) gene.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_r-pcr-012_04.png?v=1775530752\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n\u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/QPS-201.pdf?v=1775800537\" target=\"_blank\"\u003e Download Manual \u003c\/a\u003e \n  \n  \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/QPS-2_MSDS.pdf?v=1775800537\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e \n  \n  \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Toyobo","offers":[{"title":"250U","offer_id":52417385103541,"sku":"HSTTX-101","price":220.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/HSTTX-101_85e7f359-74da-4265-8b1a-52bda3bf1e84.jpg?v=1777516951","url":"https:\/\/biofargo.com\/products\/hot-start-ttx-dna-kit","provider":"Biofargo","version":"1.0","type":"link"}