Specification

Full name	Histone H3 (triMethyl-Lys27) rabbit monoclonal antibody
Alternative names	50 μl/100 μl
Reactivity	rabbit monoclonal
Applications	WB, IHC, IF
Host	Rabbit
Clone type	rabbit monoclonal
Target Background	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
Swissprot No	P68431
Gene Accession	8350
WB Predicted band size
WB Positive control	P290639-WB-1-P
WB Recommended dilution	15 kDa
IHC predicted cell location	Predicted cell location: Nucleus
IHC positive control	Positive control: Human lung cancer
IHC Recommed dilution	Recommended dilution: 50-100

Storage

-20°C, 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA. Avoid repeated freeze-thaw cycles.

P290639-WB-1-P

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using P290639(Histone H3 (triMethyl-Lys27) Antibody) at dilution 1/50