{"product_id":"high-sensitivity-probe-qpcr-master-mix-universal-starlighter","title":"High Sensitivity Probe qPCR Master Mix (Universal)——StarLighter","description":"\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\n\u003cp\u003eUltra-sensitive probe-based qPCR detection places high demands on the reagent: when the starting template is extremely low, such as in single-cell detection or amplification of picogram-level genomic DNA, non-specific amplification is a serious concern and can compromise the reliability of the results.\u003c\/p\u003e\n\n\u003cp\u003eStarLighter High Sensitivity Probe qPCR Master Mix (Universal) is a probe qPCR master mix based on an \u003cstrong\u003eultra-high-performance hot-start genetically engineered Taq Pro DNA polymerase\u003c\/strong\u003e configuration. The optimized buffer reaction system effectively \u003cstrong\u003einhibits non-specific amplification\u003c\/strong\u003e and greatly increases the amplification efficiency of qPCR, making it suitable for \u003cstrong\u003eultra-sensitive qPCR detection\u003c\/strong\u003e. This product has been verified by \u003cstrong\u003eanimal embryo sex detection\u003c\/strong\u003e and can efficiently amplify genomic DNA with as little as \u003cstrong\u003e10 pg of starting template\u003c\/strong\u003e, supporting demanding applications such as \u003cstrong\u003esingle-cell detection\u003c\/strong\u003e and \u003cstrong\u003elow copy gene detection\u003c\/strong\u003e.\u003c\/p\u003e\n\n\u003cp\u003eFor laboratories performing gene expression analysis or low copy gene detection, this high sensitivity probe qPCR master mix delivers reliable results across a broad range of starting template amounts, from ultra-low concentration to routine samples.\u003c\/p\u003e\n\n\u003ch2\u003eSpecification\u003c\/h2\u003e\n\n\u003cdiv style=\"overflow-x:auto;-webkit-overflow-scrolling:touch;\"\u003e\n  \u003ctable style=\"border-collapse:collapse;width:100%;min-width:560px;\"\u003e\n    \u003cthead\u003e\n      \u003ctr\u003e\n        \u003cth style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;font-weight:600;white-space:nowrap;\"\u003eCatalog No.\u003c\/th\u003e\n        \u003cth style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;font-weight:600;white-space:nowrap;\"\u003eSize\u003c\/th\u003e\n        \u003cth style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;font-weight:600;white-space:nowrap;\"\u003ePackaging\u003c\/th\u003e\n      \u003c\/tr\u003e\n    \u003c\/thead\u003e\n    \u003ctbody\u003e\n      \u003ctr\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003eFS-Q3002\u003c\/td\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003e5 ml\u003c\/td\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003e4 × 1.25 ml per box\u003c\/td\u003e\n      \u003c\/tr\u003e\n      \u003ctr\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003eFS-Q3001-S\u003c\/td\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003e1 ml\u003c\/td\u003e\n        \u003ctd style=\"border:1px solid #ccc;padding:8px 10px;text-align:left;vertical-align:top;\"\u003e1 tube per box\u003c\/td\u003e\n      \u003c\/tr\u003e\n    \u003c\/tbody\u003e\n  \u003c\/table\u003e\n\u003c\/div\u003e\n\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eExtremely sensitive, suitable for single-cell detection\u003c\/strong\u003e — in bovine embryo sex identification with a low starting template amount, the Ct value is 2–4 cycles ahead of that of a competitor product.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAmplifies genomic DNA from as little as 10 pg of starting template\u003c\/strong\u003e — verified by animal embryo sex detection.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAntibody-blocked hot start\u003c\/strong\u003e — the polymerase is blocked with antibodies, and enzyme activity is restored at above 90 °C for 30 seconds, effectively inhibiting non-specific amplification.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eUltra-high-performance hot-start genetically engineered Taq Pro DNA polymerase\u003c\/strong\u003e with an optimized buffer reaction system — greatly increases the amplification efficiency of qPCR.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003e5'→3' DNA synthesis with 5'→3' exonuclease activity\u003c\/strong\u003e and no 3'→5' proofreading exonuclease activity, generating 3'-dA protruding ends in the amplified product.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFast amplification speed of 1–10 s\/kb\u003c\/strong\u003e — compatible with the standard and rapid modes of qPCR instruments.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eExcellent linear range\u003c\/strong\u003e — with human genomic DNA serially diluted from 0.0064 ng to 100 ng, an excellent linear relationship is observed between the log value of the template amount and the Ct value; 5-fold serial dilutions effectively amplify ultra-low concentration templates.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eApplication\u003c\/h2\u003e\n\n\u003cp\u003eStarLighter High Sensitivity Probe qPCR Master Mix (Universal) is suitable for:\u003c\/p\u003e\n\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eGene expression analysis\u003c\/strong\u003e — accurate quantification of target genes.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eLow copy gene detection\u003c\/strong\u003e — reliable detection of ultra-low concentration templates.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSNP and allele typing\u003c\/strong\u003e — SNP detection and genotyping assays.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eValidation of sequencing and microarray results\u003c\/strong\u003e — confirmatory real-time PCR.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat is StarLighter High Sensitivity Probe qPCR Master Mix (Universal)?\u003c\/summary\u003e\n  \u003cp\u003eIt is a probe qPCR master mix based on an ultra-high-performance hot-start genetically engineered Taq Pro DNA polymerase configuration with an optimized buffer reaction system, designed for ultra-sensitive qPCR detection.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eHow sensitive is this master mix?\u003c\/summary\u003e\n  \u003cp\u003eIt can efficiently amplify genomic DNA with as little as 10 pg of starting template and is extremely sensitive, suitable for single-cell detection.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eHow does the hot-start mechanism work?\u003c\/summary\u003e\n  \u003cp\u003eThe polymerase is blocked with antibodies. Enzyme activity is restored at above 90 °C for 30 seconds, which effectively inhibits non-specific amplification during reaction setup.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat polymerase is used in this master mix?\u003c\/summary\u003e\n  \u003cp\u003eIt uses an ultra-high-performance hot-start genetically engineered Taq Pro DNA polymerase that catalyzes DNA synthesis in the 5'→3' direction, has 5'→3' exonuclease activity, and has no 3'→5' proofreading exonuclease activity. The amplified product has 3'-dA protruding ends.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat is the amplification speed?\u003c\/summary\u003e\n  \u003cp\u003eThe amplification speed is 1–10 s\/kb, and the master mix is compatible with the standard and rapid modes of qPCR instruments.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat is the linear range of this master mix?\u003c\/summary\u003e\n  \u003cp\u003eUsing human genomic DNA serially diluted from 0.0064 ng to 100 ng, there is an excellent linear relationship between the log value of the template amount and the Ct value. The reagent effectively amplifies ultra-low concentration templates in 5-fold serial dilutions (100 ng to 0.0064 ng).\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eHow does it perform in embryo sex detection?\u003c\/summary\u003e\n  \u003cp\u003eIn the identification of the sex of bovine embryos with a low starting template amount, this master mix showed higher sensitivity, with a Ct value 2–4 cycles ahead of that of a competitor product.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat applications is this master mix suitable for?\u003c\/summary\u003e\n  \u003cp\u003eIt is suitable for gene expression analysis, low copy gene detection, SNP and allele typing, and validation of sequencing and microarray results.\u003c\/p\u003e\n\u003c\/details\u003e\n\n\u003cdetails\u003e\n  \u003csummary style=\"cursor:pointer;font-weight:600;\"\u003eWhat are the available sizes?\u003c\/summary\u003e\n  \u003cp\u003eThe master mix is available as catalog No. FS-Q3002 (5 ml, packaged as 4 × 1.25 ml per box) and FS-Q3001-S (1 ml, packaged as 1 tube per box).\u003c\/p\u003e\n\u003c\/details\u003e\n","brand":"Qihengxing","offers":[{"title":"1 ml (1 tube\/box)","offer_id":53196585894069,"sku":"FS-Q3001-S","price":0.0,"currency_code":"USD","in_stock":false},{"title":"5 ml (4 × 1.25 mL\/box)","offer_id":53196585926837,"sku":"FS-Q3002","price":0.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/fs-q3002-english-orange-logo-1500x1500.jpg?v=1788319840","url":"https:\/\/biofargo.com\/products\/high-sensitivity-probe-qpcr-master-mix-universal-starlighter","provider":"Biofargo","version":"1.0","type":"link"}