Description

Abbexa's High Sensitive IHC Detection Kit is a rapid, easy to use, and versatile IHC detection system that utilizes polymer detection method technology for increased sensitivity in IHC. This new polymer technology has major advantages compared to conventional IHC systems. This kit amplifies the signal of both mouse and rabbit primary antibodies. Background noise due to non-specific binding to endogenous biotin molecules is prevented since this kit does not use a biotin/avidin based system, and so background noise due to non-specific binding to endogenous biotin is eliminated.

Kit components:

Hydrogen Peroxide Blocking Reagent buffer: 5 ml
Blocking Reagent: 5 ml
HRP Polymer: 5 ml
DAB Substrate Reagent: 5 ml
DAB Chromogen Reagent: 100 µl

Specification

Tested Applications	IHC
Storage	Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
Availability	Shipped within 7-12 working days.
Note	This product is for research use only.
Directions for use	De-wax and rehydrate tissue section; wash with PBS/TBS 3 times, 2 minutes each time. Carry out appropriate pretreatment or incubate with digestive enzymes if this is required for the primary antibody; wash with PBS/TBS 3 times, 2 minutes each time. Incubate slide in Hydrogen Peroxide Blocking Reagent for 10 minutes. Wash with PBS/TBS 3 times, 2 minutes each time. Apply Blocking Reagent and incubate for 5 minutes. Wash with PBS/TBS 3 times, 2 minutes each time. This step can be omitted if primary antibodies are diluted in buffers containing normal goat serum. Apply primary antibody and incubate according to the manufacturer's recommended protocol. Wash with PBS/TBS 3 times, 2 minutes each time. Apply the HRP Polymer (50 µl per slide) and incubate for 30 minutes. Wash with PBS/TBS 3 times, 2 minutes each time. Add 20 µl of DAB Chromogen to 1 ml of DAB Substrate. Mix by swirling and apply to tissue (50 µl per slide). Incubate for 3-5 minutes. Wash with PBS/TBS 3 times, 2 minutes each time. Counterstain and coverslip using a permanent mounting media.

High Sensitive IHC Detection Kit for Mouse and Rabbit Primary Antibody