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Hieff NGS Ultima Dual-mode RNA Library Prep Kit is a total RNA sequencing library construction kit for the Illumina and MGI sequencing platform, including RNA fragmentation reagents, reverse transcription reagents, conventional and strand-specific ds-cDNA synthesis reagents, and library amplification reagents. The sequencing library can be constructed followed by the mRNA purification kit or rRNA removal kit. The two-strand synthesis module is equipped with two buffers to meet the need for a conventional library or strand-specific library. Among them, dTTP is replaced with dUTP in the strand-specific two-strand synthesis Buffer, so dUTP can be added to the second strand of cDNA. The high-fidelity DNA polymerase used in this kit cannot amplify the DNA template containing uracil, achieving strand specificity. All reagents provided have undergone strict quality control and functional verification, ensuring the stability and reproducibility of library construction to the greatest extent.
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