{"product_id":"hieff-ngs™-onepot-pro-dna-fragmentation-module-end-repair-and-da-tailing-plus-12619es","title":"OnePot Pro DNA Fragmentation Module (end repair and dA-tailing plus)-12619ES","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eOnePot Pro DNA Fragmentation Module provides a new-generation enzymatic fragmentation reagent  designed for Illumina® and MGI® high-throughput sequencing platforms . This product simplifies the operation process compared with the complex mechanical interruption and also greatly reduces the time and cost by performing fragmentation, end repair and dA-tailing of dsDNA in one reaction.  The fragmentation products can immediately for adaptor ligation with no cleanup steps by using Fast-Pace DNA Ligation Module (Cat#12607) or Novel DNA Ligation Module (Cat#12626). OnePot Pro DNA Fragmentation Module can work with a broad range of DNA inputs (animal, plant and microbial genomes), ranging from 500 pg-1μg in library preparation. \u003c\/p\u003e\n\u003ch3\u003ePackage Information\u003c\/h3\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003eNo.\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003eName\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e12619ES24\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e12619ES96\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e12619-A\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003eSmearase™ Buffer\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e240 μL\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e960 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e12619-B\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003eSmearase™ Enzyme\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e120 μL\u003c\/td\u003e\n\u003ctd style=\"width: 25%; text-align: center; vertical-align: middle;\"\u003e480 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eNote:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e12619 is a part of Hieff NGS® OnePot Pro DNA Library Prep Kit (Cat:12205).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe Smearase™ Enzyme is mixed with enzymes involved in end repair and dA-tailing.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003eOnePot Pro DNA Fragmentation Module is shipped with ice pack and can storage at -20℃ for 1 year.\u003c\/p\u003e\n\u003ch2\u003eCautions\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eThis product is compatible with a range of 500 pg - 1 μg input DNA. High quality input DNA (A260\/A280 = 1.8-2.0) is highly recommended.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHigh concentration of metal ion chelator or other salt remained in Input DNA may affect subsequent experiments, it is recommended to dilute DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0).\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eTable 1. Guideline for choosing the fragmentation time of conventional genomic DNA\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003eMain peak size of the insert fragment\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003eFragmentation time\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003eOptimization range\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e600 bp\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e8 min\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e6-12 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e350 bp\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e10 min\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e8-14 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e250 bp\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e12 min\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e10-15 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e200 bp\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e15 min\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e13-18 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e150 bp\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e15 min\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e15-25 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003eTable 2. Guideline for choosing the fragmentation time of FFPE DNA\u003c\/p\u003e\n\u003ctable style=\"width: 90%; border-collapse: collapse;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003eMain peak size of the insert fragment\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003eFragmentation time\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003eDIN*\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e250 bp\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e9-13 min\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e\u0026gt; 8.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e250 bp\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e8-11 min\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e6.5-8.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e250 bp\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e4-8 min\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e4.2-6.5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e250 bp\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e3-6 min\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e2.5-4.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e250 bp\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e9-13 min\u003c\/td\u003e\n\u003ctd style=\"vertical-align: middle; width: 33.3333%; text-align: center;\"\u003e\u0026gt; 8.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cstrong\u003eNote: *DIN is DNA Integrity Number, which a method to define the degree of FFPE DNA degradation by using Agilent 2200.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eFor conventional high-quality genomic DNA, fragmentation time refers to Table 1. This product is compatible with various samples with different GC content and has minimal bias. For the FFPE samples with different degrees of degradation, the fragmentation time refers to Table 2. Customers need to fine-tune the recommended fragmentation time in their own experimental system to achieve the best results.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eThe DNA can be digested to the required size according to the recommended fragmentation time. In order to ensure the high-quality and stable fragmentation effect, the fragmentation process should be operated on ice.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eFor your safety and health, please wear personal protective equipment (PPE), such as laboratory coats and disposable gloves, when operating with this product.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003ch3\u003eSafety Data Sheet\u003c\/h3\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12619-COMPONENT_A-MSDS-HB220428.pdf?v=1749693685\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003e12619-COMPONENT A-MSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12619-COMPONENT_B-MSDS-HB220428.pdf?v=1749693685\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003e12619-COMPONENT B-MSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch3\u003eManuals\u003c\/h3\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EN-12619-Hieff_NGSTM_OnePot_Pro_DNA_Fragmentation_Reagent-HB220407.pdf?v=1749693685\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eEN-12619 OnePot Pro DNA Fragmentation Reagent-HB220407.pdf\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasenbio","offers":[{"title":"24T","offer_id":45487546269877,"sku":"12619ES24","price":222.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":45487546302645,"sku":"12619ES96","price":716.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/1_04139f72-8806-46e5-af82-154af7c96ad2.jpg?v=1776395592","url":"https:\/\/biofargo.com\/products\/hieff-ngs%e2%84%a2-onepot-pro-dna-fragmentation-module-end-repair-and-da-tailing-plus-12619es","provider":"Biofargo","version":"1.0","type":"link"}