{"product_id":"hieff-ngs™-mrna-isolation-master-kit-v2","title":"Hieff NGS™ mRNA Isolation Master Kit V2","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHieff NGS™ mRNA Isolation Master Kit V2 is an upgraded magnetic bead kit specifically designed for purifying mRNA. The mRNA Capture beads are micron-sized paramagnetic microspheres conjugated with Oligo (dT). They combine with the mRNA carrying a poly (A) tail to separate and purify the mRNA from 10 ng to 4 μg of total RNA with good integrity.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProduct Line\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eMagnetic Bead\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eStarting Material\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eRNA (10 ng – 4 μg)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003emRNA Isolation Method\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eOligo dT magnetic beads\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eApplication\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eRNA enrichment\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eWide compatibility: \u003c\/strong\u003eCompatible with total RNA input amounts ranging from 10 ng to 4 μg, and compatible with total RNA library preparation kits from different brands\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eInhibitor tolerance: \u003c\/strong\u003eHigh mRNA yield and low rRNA residual for samples containing inhibitors, such as herbal medicines, polysaccharide- and polyphenol-rich samples, and tumor tissues.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh mRNA purity: \u003c\/strong\u003eSuitable for eukaryotic samples with poly(A) tails.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eComponents\u003c\/h2\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eStorage\u003cbr\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThis product should be stored at 2~8℃ for 12 months.\u003c\/p\u003e\n\u003ch2\u003eFigures\u003cbr\u003e\n\u003c\/h2\u003e\n\u003ch3\u003e1. Workflow\u003c\/h3\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12629-1_1024x1024_acc8a795-2fe8-46e6-9699-3df126721946.jpg?v=1777008738\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 1. mRNA isolation workflow\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch3\u003e2. Wide input range compatibility\u003c\/h3\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12629-2_1024x1024_586ae141-adfb-480f-8710-7c7fdf04248d.jpg?v=1777008864\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 2. rRNA Residual Levels Across Different Input Amounts\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eLibrary preparation was performed using 12629 and RNA Library Preparation Kit (Cat#12308, with both strand-specific and non-strand-specific protocols) on conventional RNA samples with varying input amounts. The rRNA residual rate was significantly lower compared to Supplier V*.\u003c\/p\u003e\n\u003ch3\u003e3. rRNA Removal Across Compatible Species\u003c\/h3\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12629-3_1024x1024_5b6d91f0-143d-464a-84af-dc814ea06620.jpg?v=1777008864\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 3. rRNA Residual Levels Across Different Species\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eLibrary preparation was performed using the 12629 and RNA Library Preparation Kit (Cat#12308, strand-specific protocol). The rRNA residual rate was significantly lower compared to Supplier B*.\u003c\/p\u003e\n\u003ch3\u003e4. Stability\u003c\/h3\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12629-4_1024x1024_f2e25aae-6685-4251-9f6e-0eec3aa245e3.jpg?v=1777008865\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFigure 4. Stability Test\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e12629 stored at 4°C for 2 weeks and 37°C for 2 weeks, combined with RNA library preparation kit(Cat#12308), shows consistent library yield and rRNA depletion using 1 µg of 293 RNA for strand-specific library preparation.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/EN-12629-MSDS-HB250623.pdf?v=1777010957\"\u003e\u003cstrong\u003eMSDS\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/12629ES-Manual-Ver.EN20241204.pdf?v=1777010956\"\u003e\u003cstrong\u003eManuals\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e","brand":"Yeasenbio","offers":[{"title":"8T","offer_id":52457751675061,"sku":"12629ES08","price":65.0,"currency_code":"USD","in_stock":true},{"title":"24T","offer_id":52457751707829,"sku":"12629ES24","price":95.0,"currency_code":"USD","in_stock":true},{"title":"96T","offer_id":52457751740597,"sku":"12629ES96","price":305.0,"currency_code":"USD","in_stock":true},{"title":"96T(automation)","offer_id":52457751773365,"sku":"12629ES97","price":349.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/iShot_2025-12-01_12.42.00.jpg?v=1777010156","url":"https:\/\/biofargo.com\/products\/hieff-ngs%e2%84%a2-mrna-isolation-master-kit-v2","provider":"Biofargo","version":"1.0","type":"link"}