Description

Fast-Pace DNA Cyclization Kit for MGI is a single-strand cyclization kit specifically designed for MGI platforms. The use of high-quality enzymes and optimized Buffer significantly reaction efficiency, enabling the entire cyclization and digestion process to be completed in less than 30 minutes. This kit is suitable for all standard single-barcode libraries for MGI platforms and is not limited to different MGI sequencing platforms except for the limitations of the library-prep reagents.

Specifications

Product Type	NGS Library Preparation
Product Line	DNA library Preparation
Input DNA	The recommended Input DNA is 1 pmol, if the PCR product is insufficient, down to 0.5 pmol
For Use With (Application)	Single Barcode DNA Cyclization Kit for MGI
For Use With (Equipment)	MGI Platforms
Quantity	16/96 Reactions

Features

Wide range of use: compatible with mainstream library preparation kits (KAPA, etc.)
Fast cyclization: a 1-hour rapid cyclization
High cyclization efficiency: the cfDNA cyclization efficiency>30%,other sample types>40%

Applications

To prepare a single-stranded circular DNA library dedicated to the MGI high-throughput sequencer

Components

Components No.	Name	13341ES16 (16T)	13341ES96 (96T)
13341-A	Splint Oligo	96 μL	576 μL
13341-B	Splint Buffer	240 μL	2×720 μL
13341-C	Ligase	80 μL	480 μL
13341-D	Digestion Buffer	128 μL	768 μL
13341-E	Digestion Enzyme	32 μL	192 μL

Figures

Figure 1. Comparison of cyclization efficiency of PCR-free library preparation for different fragment lengths.

Method: The PCR-free libraries were prepared with Cat#12202 PCR-free library prep kit.
Results: The library cyclization efficiency of the different library fragments Cat#13341 was higher than that of Supplier M*

Storage

-25℃ ~ -15℃ storage, valid for one year.

Documents

Safety Data Sheet

13341-COMPONENT A-MSDS-HB220420.pdf

13341-COMPONENT B-MSDS-HB220420.pdf

13341-COMPONENT C-MSDS-HB220420.pdf

13341-COMPONENT D-MSDS-HB220420.pdf

13341-COMPONENT E-MSDS-HB220420.pdf

Manuals

13341_Manual_HB230112_EN.pdf

Fast-Pace DNA Cyclization Kit for MGI-13341ES