Hieff miRNA Universal qPCR SYBR Master Mix

MicroRNA, a class of non-coding RNAs with a length of about 22 nt, is widely concerned and plays a very important role in regulating gene expression in plants and animals. This kit uses the SYBR Green I chimeric fluorescence method for the quantitative detection of miRNA fluorescence. The 2×Hieff miRNA Universal qPCR SYBR Master Mix is a new generation of premix fluorescent quantitative PCR detection reagent specially developed for the quantitative detection of miRNA. It contains special ROX Passive Reference Dye. Suitable for most qPCR instruments, there is no need to adjust the concentration of ROX on different instruments, just add primers and templates in the preparation of the reaction system can be amplified. The DNA Polymerase uses chemically modified heat-activated Polymerase, combined with a special Buffer system, to make the reaction more specific, more sensitive, and can be accurately quantified in a wider range.
This product is recommended for use with our Hifair miRNA 1st Strand cDNA Synthesis Kit (Tailing) (Cat# 11148) to obtain optimal experimental results.