Synonym(s): 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt, HEPES, Sodium Salt

Description

HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate.

Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.

Specifications

Item	Value
Chemical Name	HEPES Sodium Salt 99.0% High Purity Grade
Appearance	White Powder
CAS No.	75277-39-3
Molecular Formula	C8H17N2O4SNa
Molecular Weight	260.30
Grade	High Purity Grade
Assay( Titration, Dry Basis)	≥99.0%
Loss on Drying (110oC, 3hr)	≤3.0%
Infrared	Complies
pH (1% Di H2O, 25℃)	9.5 ~ 10.5
Solubility(0.1M,Water)	Clear Colorless Solution
A260 (1M water)	≤0.2
A280 (1M water)	≤0.2
A440 (1M water)	≤0.2
Heavy Metals(as Pb)	≤5ppm
Storage	Store at Room Temperature. Protect from Moisture.

Application

HEPES buffer finds application in:

Most studies with metal ions
Substitute for Tris and phosphate in studies with metal ions
Grinding buffer in plant studies
Running buffer in gel electrophoresis
Buffer in electroporation studies
Bradford or bicinchoninic acid (BCA) assays
Extraction buffer to prevent damage of proteins in red blood cells

It has been used:

as a buffer component in the preparation of a low sucrose buffer
as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions

Features

Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Highly soluble in water
Negligible metal ion binding
Less toxic to cells than other buffers such as Tris and phosphate
Stable in a wide pH range

Documents

INFO

COA

SDS

Disclaimer: For laboratory research use only.

HEPES Sodium Salt 99.0% High Purity Grade