Description

HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate.

Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.

Specifications

Total Product Size	HB0265 (50g) HB0265 (250g) HB0265 (1kg)
Shipping Conditions	RT
UNSPSC Code	12161700
UNSPSC Category	Buffers
Grade	ACS
CAS	[75277-39-3]
DG	No
Storage	18 to 25℃
Sterile	Yes

Application

HEPES buffer finds application in:

Most studies with metal ions
Substitute for Tris and phosphate in studies with metal ions
Grinding buffer in plant studies
Running buffer in gel electrophoresis
Buffer in electroporation studies
Bradford or bicinchoninic acid (BCA) assays
Extraction buffer to prevent damage of proteins in red blood cells

It has been used:

as a buffer component in the preparation of a low sucrose buffer
as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions

Features

Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
Highly soluble in water
Negligible metal ion binding
Less toxic to cells than other buffers such as Tris and phosphate
Stable in a wide pH range

Documents

INFO

COA

SDS

Disclaimer: For laboratory research use only.

HEPES, Sodium Salt