{"product_id":"hepes-99-5-ultrapure-grade","title":"HEPES Ultrapure Grade, ≥99.5% — Low UV Absorbance","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHEPES Ultrapure Grade is a refined version of the standard HEPES buffer, designed for high-sensitivity biological and molecular workflows. It delivers the same reliable buffering performance as Biochemical Grade, but with significantly reduced UV-absorbing impurities — minimizing interference in optical and enzyme-based assays.\u003c\/p\u003e\n\u003cp\u003eBoth Biofargo's Biochemical and Ultrapure grades are supplied at ≥99.5% assay purity. The difference is refinement: Ultrapure Grade is further processed to achieve lower absorbance at A260 and A280, indicating reduced organic contaminants. This makes a measurable difference in applications where background signal, enzyme stability, or cell viability is critical — such as PCR, fluorescence imaging, and sensitive cell-based assays.\u003c\/p\u003e\n\u003cp\u003eThe product forms a clear, colorless solution and maintains stable buffering capacity near physiological pH (pKa 7.6 at 20°C), with tight control of sulfate, residue, and UV absorbance.\u003c\/p\u003e\n\u003cp\u003eFor routine cell culture, general protein work, and standard buffer preparation where UV background is not a concern, our \u003ca href=\"https:\/\/biofargo.com\/products\/hepes-free-acid-biofargo?variant=44909483491509\"\u003e\u003cstrong\u003eHEPES Biochemical Grade\u003c\/strong\u003e\u003c\/a\u003e is the more cost-effective option.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable style=\"border-collapse: collapse; width: 90%;\" border=\"1\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eCAS No.\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e7365-45-9\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e7365-45-9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eMolecular Formula\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eC₈H₁₈N₂O₄S\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eC₈H₁₈N₂O₄S\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eMolecular Weight\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e238.31\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e238.31\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003ePurity\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≥99.5%\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≥99.5%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eGrade\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eBiochemical Grade\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eUltrapure Grade\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eSolubility (1M in H₂O)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eColorless, clear\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eColorless, clear\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eAppearance\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eWhite powder\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eWhite powder\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003epKa (20°C)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e7.4 – 7.7\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e7.4 – 7.7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eSulfate\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≤0.05%\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≤0.05%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eLoss on Drying\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≤0.5%\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≤0.3%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eResidue on Ignition\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e—\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≤0.1%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eHeavy Metals (Pb)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≤5 ppm\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eHeavy Metals (Fe)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≤3 ppm\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eUV Absorbance (250 nm, 1M)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≤0.10\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≤0.10\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eUV Absorbance (260 nm, 1M)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≤0.05\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≤0.05\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eUV Absorbance (280 nm, 1M)\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≤0.04\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003e≤0.04\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eRecommended Application\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eGeneral buffers\u003c\/td\u003e\n\u003ctd style=\"width: 33.3333%; text-align: center;\"\u003eCell culture \/ PCR \/ sensitive assays\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eLow UV Absorbance for High Sensitivity Applications\u003c\/strong\u003e — Reduced A260\/A280 values indicate lower levels of organic impurities, minimizing interference in fluorescence, PCR, and imaging workflows.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eHigh Chemical Purity (≥99.5%) \u003c\/strong\u003e— Ensures consistent buffering performance across experimental batches while meeting standard reagent-grade requirements.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eOptimized for Optical and Enzyme-Based Systems\u003c\/strong\u003e — Lower background signal improves assay accuracy in fluorescence detection, enzyme kinetics, and molecular biology experiments.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eStable Physiological Buffering Capacity\u003c\/strong\u003e — Maintains pH stability around physiological conditions (pKa ~7.6), ideal for biological systems.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eControlled Inorganic Residues\u003c\/strong\u003e — Tight limits on sulfate content and residue on ignition contribute to improved solution clarity and reproducibility.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eExcellent Solubility and Clarity\u003c\/strong\u003e — Forms a clear, colorless solution suitable for optical and analytical applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eCell Culture Systems\u003c\/strong\u003e — Suitable for sensitive cell-based assays where reduced impurity levels help maintain cell viability and consistency.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003ePCR \/ qPCR \/ Molecular Biology \u003c\/strong\u003e— Lower UV absorbance minimizes interference with nucleic acid detection and amplification reactions.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eFluorescence Imaging \u0026amp; Optical Assays\u003c\/strong\u003e — Reduced background signal improves sensitivity in fluorescence-based detection systems.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eEnzyme Reactions \u003c\/strong\u003e— Ideal for kinase assays, polymerase reactions, and other enzyme-sensitive applications.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eElectrophysiology \u0026amp; Neuroscience Research\u003c\/strong\u003e — Suitable for applications sensitive to oxidative or impurity-related interference.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eAdvanced Research \u0026amp; Process Development \u003c\/strong\u003e— Applicable in workflows requiring higher purity buffering systems and reproducibility.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety Disclaimer\u003c\/h2\u003e\n\u003cp\u003eThis product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the SDS provided by Biofargo for detailed handling, storage, hazard, and disposal information. Use appropriate PPE when handling. Dispose of waste in accordance with local, state, and federal regulations.\u003c\/p\u003e\n\u003ch2\u003eFAQ\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eWhen should I use Ultrapure Grade HEPES instead of Biochemical Grade?\u003c\/strong\u003e\u003cbr\u003eChoose Ultrapure Grade when UV-absorbing impurities could interfere with your results — for example in PCR and qPCR, fluorescence imaging, optical and absorbance-based assays, sensitive enzyme kinetics, or cell culture work where cell viability is critical. For routine buffer preparation, general cell culture, and standard protein work, \u003cstrong\u003e\u003ca href=\"https:\/\/biofargo.com\/products\/hepes-free-acid-biofargo?variant=44909483491509\"\u003eBiochemical Grade\u003c\/a\u003e\u003c\/strong\u003e is a more cost-effective choice that delivers the same ≥99.5% assay purity. (See our HEPES Biochemical Grade page.)\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDoes Ultrapure Grade HEPES have higher purity than Biochemical Grade?\u003c\/strong\u003e\u003cbr\u003eBoth grades are supplied at ≥99.5% assay purity. The distinction is in refinement: Ultrapure Grade has lower UV absorbance (A250\/A260\/A280), reflecting reduced organic impurities, plus tighter control of residue. So the difference is less about the headline purity number and more about the impurity profile relevant to sensitive optical and enzymatic applications.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eWhy does low UV absorbance matter?\u003c\/strong\u003e\u003cbr\u003eUV-absorbing impurities in a buffer add background signal to any measurement made by absorbance or fluorescence. In PCR, nucleic acid quantification, and fluorescence imaging, that background can reduce sensitivity and affect data quality. A buffer with low UV absorbance keeps the buffer itself from interfering with the measurement.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eIs Ultrapure Grade HEPES suitable for cell culture?\u003c\/strong\u003e\u003cbr\u003eYes. Ultrapure Grade is well suited to sensitive cell-based assays, where reduced impurity levels help support cell viability and experimental consistency. For routine cell culture where impurity sensitivity is not a concern, Biochemical Grade also works well and costs less.\u003c\/p\u003e\n\u003ch2\u003ePublications\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eRecent research using HEPES buffer\u003c\/strong\u003e (\u003cem\u003esource: PubMed\u003c\/em\u003e)\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eCorazolla, E.M., et al. 2026. HEPES in Cell Culture Alters the Multi-Omics Profile Exhibited by Gaucher Disease Fibroblasts.\u003cem\u003e J Cell Biochem\u003c\/em\u003e 127(1), e70080. DOI — Study examining how HEPES in culture media influences cellular metabolism and diagnostic outcomes; relevant context for choosing buffer grade in sensitive cell work.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eMerli, M., et al. 2022. Pectin-based bioinks for 3D models of neural tissue produced by a pH-controlled kinetics. Front \u003cem\u003eBioeng Biotechnol\u003c\/em\u003e 10, 1032542. DOI — HEPES buffer used as a component in optimizing pH-controlled bioink formulations for 3D-bioprinted neural cell models.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eYeung, S.Y., et al. 2022. Reversible Self-Assembled Monolayers with Tunable Surface Dynamics for Controlling Cell Adhesion Behavior. \u003cem\u003eACS Appl Mater Interfaces\u003c\/em\u003e 14(37), 41790–41799. DOI — HEPES buffer solution (pH 8) used as the medium for preparing reversible self-assembled monolayers for cell adhesion studies.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Nanjing Search Biotech","offers":[{"title":"25g","offer_id":52476913418421,"sku":"BF0264-25g","price":28.0,"currency_code":"USD","in_stock":true},{"title":"50g","offer_id":52476913451189,"sku":"BF0264-50g","price":42.0,"currency_code":"USD","in_stock":true},{"title":"100g","offer_id":52476913483957,"sku":"BF0264-100g","price":83.0,"currency_code":"USD","in_stock":true},{"title":"250g","offer_id":52476913516725,"sku":"BF0264-250g","price":158.0,"currency_code":"USD","in_stock":true},{"title":"500g","offer_id":52476913549493,"sku":"BF0264-500g","price":300.0,"currency_code":"USD","in_stock":true},{"title":"1000g","offer_id":52476913582261,"sku":"BF0264-1000g","price":389.0,"currency_code":"USD","in_stock":true},{"title":"5000g","offer_id":52476913615029,"sku":"BF0264-5000g","price":1429.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/HEPES_99.5_Biochemical_Grade.jpg?v=1765788809","url":"https:\/\/biofargo.com\/products\/hepes-99-5-ultrapure-grade","provider":"Biofargo","version":"1.0","type":"link"}