Synonym(s): N-(2-Hydroxyethyl)piperazine-N′-(4-butanesulfonic acid)

Description

HEPBS (N-(2-Hydroxyethyl)piperazine-N′-(4-butanesulfonic acid)) is a high-purity Good’s buffer widely used in biochemical and molecular biology research. With an assay of ≥99%, this biochemical grade reagent provides excellent pH stability in the near-neutral range, making it well suited for sensitive biological reactions. HEPBS exhibits low metal ion binding, minimal interference with enzymatic activity, and high chemical stability, ensuring reliable and reproducible experimental results.

This product is manufactured under strict quality control standards and supplied as a white crystalline powder, suitable for preparation of precise buffer systems in research laboratories.

Specifications

Item	Value
Chemical Name	N-(2-Hydroxyethyl)piperazine-N'-(4-butanesulfonic acid)
Appearance	White Crystalline Powder
CAS No.	161308-36-7
Molecular Formula	C10H22N2O4S
Molecular Weight	266.36
Grade	Biochemical Grade
Assay (Titration)	≥99%
Moisture	≤0.5%
Infrared Spectrum	Conforms to Specification
Residue on Ignition	≤0.1%
Iron	≤5 ppm
Lead	≤1 ppm

Features

High Purity (≥99%)

Biochemical grade quality ensures consistent buffering performance in sensitive biological and biochemical assays.
Optimal Buffering Range (pH 6.8–8.2)

Ideal for maintaining near-neutral pH conditions required for most biological reactions.
Low Metal Ion Chelation

Minimizes interference with metal-dependent enzymes and biochemical processes.
Excellent Chemical Stability

Stable during storage and solution preparation, supporting reproducibility across experiments.
Research-Grade Quality Control

Verified by infrared spectrum conformity and strict limits on moisture, residue on ignition, and heavy metals.

Applications

Preparation of biological and biochemical buffer solutions
Enzyme activity assays and protein studies
Cell-free biochemical reactions
Molecular biology workflows requiring stable pH control
General laboratory research in life science and biotechnology fields

Safety Disclaimer

This product is intended for laboratory research use only. Not for drug, human, or veterinary use. Review the Safety Data Sheet (SDS) provided by BioFargo for detailed handling, storage, hazard, and disposal information. Use appropriate personal protective equipment (PPE) including gloves, lab coat, and eye protection when handling. Dispose of waste and residual buffer in accordance with local, state, and federal regulations. Ensure proper ventilation if preparing large volumes or heating solutions.

Documents

SDS

HEPBS 99% Biochemical Grade