Description

HAYFLICK broth is enriched with horse serum, supplying biologically available cholesterol and long-chain fatty acids essential for mycoplasmal membrane biogenesis. Exogenous deoxyribonucleic acid (DNA) is incorporated as a source of purine and pyrimidine bases, which Mycoplasma spp. are unable to synthesize de novo.

Growth-associated acid or alkaline shifts are detected by the phenol red pH indicator, manifesting as a transition of the medium from red to yellow—or occasionally to purple—thus providing a rapid visual proxy for mycoplasma proliferation. Penicillin G is included to selectively suppress concomitant bacterial contaminants while sparing mycoplasmal cells.

Supplementation with a defined lipid mixture (cholesterol and fatty-acid precursors) further enhances biomass yield and colony-forming efficiency, a benefit that is especially pronounced for lipid-dependent strains such as Mycoplasma pneumoniae ATCC 23714.

Specifications

Agency	EP 2.6.7 / USP / JP
Quality Level	ISO 9001
Sterility	Sterile
-	Aseptically Filled
Form	Liquid
Feature	Ready-to-use; ICR Settle plates
packaging	1 bottle
Bottle Filling Volume	100 mL
pH	7.8 (in H2O)
Application(s)	Industrial qc
Storage Temp	2-8℃ for 6 months -20℃ -80℃ for 1 year
Suitability	Mycoplasma

Application

HAYFLICK broth is used for the general detection of mycoplasmas in pharmaceutical products, particularly in bulk vaccines, cell banks, and virus cultures.

Features

Ready-to-use convenience — no additional preparation steps are required.
Broad pharmaceutical applications — suitable for biopharmaceuticals, vaccines, cell cultures, and virus cultures.
Storage stability — can be stored at 2–8 °C for up to 6 months or at −20 °C for 1 year.
Rapid detection — a colorimetric chart provides a quick and convenient visual indication of early-stage mycoplasma proliferation.
Optimized growth performance — the buffered system supports higher colony counts while reducing the risk of mycoplasma cell death.
Enhanced visualization — a chromogenic reagent selectively stains mycoplasma, improving clarity and specificity under microscopy.
Improved NAD⁺ stability — to prevent growth inhibition caused by NAD⁺ degradation during later stages, alternative NAD⁺ analogs are co-supplemented in the culture system.

Documents

M-HAYFLICK-Y SDS

M-HAYFLICK-Y COA 01

M-HAYFLICK-Y COA 02

Hayflick broth (M-HAYFLICK-Y)