{"product_id":"hamster-primary-thymus-fibroblasts","title":"Hamster Primary Thymus Fibroblasts","description":"\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eHamster Primary Thymus Fibroblasts from \u003cem\u003eCell Biologics\u003c\/em\u003e\u003cstrong\u003e \u003c\/strong\u003eare isolated from thymus tissue of hamster. Hamster Primary Thymus Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in \u003cem\u003eCell Biologics\u003c\/em\u003e\u003cstrong\u003e \u003c\/strong\u003eCulture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10\u003csup\u003e6\u003c\/sup\u003e cells per ml and is delivered frozen. \u003c\/p\u003e\n\u003cp\u003eHamster Primary Thymus Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by \u003ci\u003eCell Biologics. \u003c\/i\u003eRepeated freezing and thawing of cells is not recommended.\u003c\/p\u003e\n\u003ch3\u003eSuggested Medium\u003c\/h3\u003e\n\u003cp\u003e Fibroblast Medium \/w Kit - 500 ml\u003c\/p\u003e\n\u003cp\u003eCatalog No. \u003cb\u003eM2267\u003c\/b\u003e\u003c\/p\u003e\n\u003ch2\u003eLaboratory Applications\u003c\/h2\u003e\n\u003cp\u003eHamster Primary Thymus Fibroblasts can be used in assays of standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.\u003c\/p\u003e\n\u003ch2\u003eStorage\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eCell Biologics\u003c\/i\u003e ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live-cell shipment is also available on request.\u003c\/p\u003e\n\u003cp\u003eNever can primary cells be kept at -20 °C.\u003c\/p\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/HM-6210_HM-6210.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eHM-6210\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/files.biofargo.com\/cell-biologics\/pdfs\/HM-6210_Cell-Culture-Protocol-CB.pdf\" type=\"application\/pdf\" target=\"_blank\"\u003e\u003cstrong\u003eCell-Culture-Protocol-CB\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003ch2\u003eDisclaimer\u003c\/h2\u003e\n\u003cp\u003eAlthough Hamster Thymus Fibroblasts are isolated from laboratory mice testing pathogen-free, investigators should handle the cells that they receive from \u003ci\u003eCell Biologics\u003c\/i\u003e with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.\u003cbr\u003e\u003c\/p\u003e\n","brand":"Cell Biologics","offers":[{"title":"Frozen Vial","offer_id":52481339588789,"sku":"HM-6210","price":510.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/11022011Media_cells_008_copy-500x500_346264e0-8fbf-46fe-ba9a-1905ae8af870.jpg?v=1778253332","url":"https:\/\/biofargo.com\/products\/hamster-primary-thymus-fibroblasts","provider":"Biofargo","version":"1.0","type":"link"}