Description

Guanidine Thiocyanate (Guanidinium thiocyanate or guanidinium isothiocyanate, GITC), a powerful protein denaturant, is most often used to inactivate endogenous RNases in the isolation of RNA from various tissues and bacteria. When used with acid-equilibrated phenol or phenol: chloroform, a solution of Guanidine Thiocyanate and beta-mercaptoethanol are very effective in disrupting both cytoplasmic and nuclear membranes while maintaining the integrity of the RNA.

Guanidine Thiocyanate is also used as a storage buffer for blood samples.

Guanidine Thiocyanate from Bio Basic has been manufactured and specially packaged to provide a higher purity nuclease-free product.

References

1. Chomczynski, P. and N. Sacchi. 1987. Anal. Biochem. 162:156-159.

2. Kedzierski, W. and J.C. Porter. 1991. BioTechniques. 10:210-214.

3. Chirgwin, J.M., et.al., 1979. Biochem. 18:5294.

4. Maniatis, T., et. al., 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Press.

Number of Containers:1

Shipping Conditions:RT

UNSPSC Code:12352200

UNSPSC Category:Biochemical Reagents

Hazard Class:8

Hazard UN:1759Hazard PG:PGIII

CAS:[593-84-0]

Grade:Ultra Pure

DG:Yes

Storage:18 to 25℃

Sterile:NoStock:Usually in Stock

Documents

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Disclaimer: For laboratory research use only.

Guanidine Thiocyanate (GITC)