Specification

Package	1 KIT
Full name	GST 4FF Sefinose(TM) Resin Kit
Applications	For the separation and purification of soluble recombinant proteins with GST tags, inclusion body proteins dissolved in high concentration denaturants cannot be purified using this medium.
Summary	Glutathione S-transferase (GST) derived from Schistosoma japonicum is a commonly used fusion label in the design and expression of recombinant proteins in genetic engineering. After fusion with GST, the recombinant protein not only improves stability and solubility, but also brings great convenience for downstream separation and purification. GST has a molecular weight of approximately 26 kDa and a strong affinity for reduced glutathione (GSH). Therefore, immobilized GSH can be used to achieve one-step affinity purification of GST fusion proteins.
Features	1. With good specificity and high affinity with GST fusion protein, the target protein with a purity of over 95% can be obtained through one-step affinity purification from bacterial fragmentation solution; 2. The GST fusion protein bound to the medium can be competitively eluted by high concentrations of reduced glutathione (GSH) (33 mM) in solution. For different GST fusion proteins, there is no need to explore the optimal GSH elution concentration, making the use of the medium simpler; 3. Renewable and reusable.
Composition	1Kit (1 ml X 10 columns + 500 ml Elution Buffer + 500 ml Binding/Wash Buffer)
Substrate	Highly cross-linked 4% agarose
Average Particle Size	90 μm（45-165 μm）
Particle Size	0
Protein Binding Ability	Approximately 5-10 mg GST labeled protein/1ml
Maximum Flow Rate	300 cm/h
Pressurization	0.3 MPa, 3 bar
Storage Buffer	20% ethanol
Component	1Kit (1 ml X 10 columns + 500 ml Elution Buffer + 500 ml Binding/Wash Buffer)

Storage

2~8°C, do not freeze

GST 4FF Sefinose(TM) Resin Kit