Description

The 5 mL Gravity Columns (total volume 6.5 mL) are economical, disposable tools designed for small-scale gravity flow protein and antibody purification, buffer exchange, and desalting. Supplied empty (no resin included), they feature durable polyethylene frits, clear graduations, and secure snap-on caps—offering flexibility, reliability, and cost-effective performance. Compact and easy to handle, these columns are ideal for rapid sample cleanup prior to SDS-PAGE, HPLC, or mass spectrometry.

Specification

Package	5/PK/25/PK
Full name	Gravity Columns, 5 ml
Applications	The resin bed volume is 2.5 ml.Supplied with caps and stoppers.
Summary	The 5ml empty, plastic, disposable columns have an internal volume of 6.5ml and is designed for small scale gravity flow purifications.
Features	Total Volume: 6.5ml Resin Volume: 2.5ml Reservoir Volume: 4ml Closure: Plastic Stopper Cap: Push in cap Frit: 1.5mm ~30μm hydrophobic polyethylene
Composition	?Columns, matching cap, frits, and rubber stoppers.
Total Volume	6.5 ml
Resin Volume	2.5 ml
Reservoir Volume	4 ml
Closure	Plastic Stopper
Cap	Snap-on cap
Frit	1.5 mm ~30 μm hydrophobic polyethylene.
Component	?Columns, matching cap, frits, and rubber stoppers.

Gravity Columns, 5 mL – FAQ

Q: What are they used for?
A: Perfect for small-scale protein and antibody purification, buffer exchange, and quick sample cleanup before downstream analysis.

Q: What samples can I load?
A: Typically cell lysates, fermentation supernatants, or antibody-containing culture media.

Q: What quality matters most?
A: Secure snap-on caps, stable frits for uniform flow, and clear columns ensure reliable and reproducible results.

Q: Any common challenges?
A: Flow control and recovery can be tricky at very small scales, but our well-designed frits and column geometry minimize dead volume and sample loss.

Q: Why choose these columns?
A: They’re compact, clean, consistent, and cost-effective—with multiple pack sizes (5 pcs / 25 pcs) for flexible lab use.

Q: Do these columns fit LabMate-style racks?
A: Yes—our 5 mL gravity columns fit most LabMate-style and universal chromatography racks designed for ~12 mm wells. If your rack uses special-size wells, please check the well diameter before purchase.

Q: Can I collect into 1.5 mL, 2 mL, or 15 mL tubes?
A: Yes. The outlet geometry allows stable positioning over microcentrifuge tubes (1.5/2 mL) as well as 15 mL conical tubes.

Q: Are these compatible with ion-exchange/affinity/gel-filtration resins?
A: Yes. The columns are supplied empty (no resin) and are compatible with common IEX, affinity (Ni-NTA, Protein A/G, GST, etc.), and size-exclusion media.

Storage

Room temperature

Gravity Columns, 5 ml