Background

CD4 is an accessory molecule involved in TCR recognition of MHC class II/peptide complexes. Expressed predominantly on T helper cells, and to a lesser extent on monocytes and dendritic cells, CD4 also serves as the entry receptor for HIV. The CD4 antibody used in NanoSep™ CD4 beads binds most thymocytes and approximately 65% of peripheral blood T cells, enabling targeted enrichment or depletion of CD4⁺ cells.

Description

NanoSep™ CD4 Magnetic Beads are 50 nm magnetic particles conjugated with anti-human CD4 monoclonal antibodies, specifically designed for the positive selection or depletion of CD4⁺ T cells from peripheral blood mononuclear cells (PBMCs). These beads enable efficient and highly specific isolation of CD4⁺ T cells with excellent recovery, purity, and viability. NanoSep™ CD4 beads are compatible with both manual and automated magnetic separation systems, including column-based platforms such as Miltenyi Biotec's MS columns and column-free systems like Applied Cells’ MARS® Bar, making them a flexible and powerful tool for downstream immunology and cell therapy research.

Key Features

High cell input capacity: Efficiently processes up to 1×10⁹ PBMCs per run
Exceptional performance:
- Recovery rate: ≥ 95%
- Purity: ≥ 98% CD4⁺ T cells
- Viability: ≥ 98% viable post-separation
Bead size: 50 nm for optimal labeling and minimal steric hindrance
Compatibility: Suitable for both column-based and column-free systems
Flexible applications: Supports positive selection or depletion protocols
Reactive species: Human
Storage: 2–8°C
Endotoxin level: < 2 EU/mL
Shelf life: 6 months

Applications

NanoSep™ CD4 beads are suitable for isolating CD4⁺ T cells for downstream applications such as:

Functional assays (e.g., proliferation, cytotoxicity)
Cytokine profiling
Co-culture experiments with B cells or dendritic cells
Immune monitoring in infectious disease and oncology research
T cell expansion and in vitro differentiation protocols

Compatible Columns and Instruments
- Column-based separation: MS, LS, and autoMACS® columns (Miltenyi Biotec)
- Column-free separation: Compatible with MARS® Bar (Applied Cells) and similar platforms

Data Figures

Documents

TL-623_Product Sheet.pdf

GMP Grade MaxSortin® CD4 Separation Magnetic Beads-T&L