Reaction Equation

L-Glutamate + H2O + NADP+ = 2-Oxoglutarate + NH3 + NADPH

Specifications

Specific Activity ( IU/mg protein ) > 10 U/mg protein
(in case of using L-Glutamate as substrates)
Contaminants
Glucose-6-phosphate dehydrogenase <0. 1%
Phosphogluconate dehydrogenase <0.5%
Glutamate dehydrogenase（NAD+） <0. 1%
Glutathione reductase <0. 1%
NADPH oxidase <0.01%

Preparation and storage

Lyophilized powder (contains no ammonium sulfate)
Store below -20ﾟC
IU per 1 mg powder is approximately 30 units.

Assay Procedure

Spectrophotometric Method:

Wavelength: 340 nm,
Light path length: 1 cm
Temperature: 25ﾟC

Pipette the following reagents into a cuvette
2.85 mL Na-Pyrophosphate buffer（0.1 mol/L, pH 9.0）
containing L-Glutamate（0.1 mol/L）
0.15 mL NADP+（10 mmol/L）
0.02 mL GlDH（NADP）（about 3 IU/mL）

Calculation

ΔA/min: The change in absorbance at 340 nm/minute

V: Total volume of reaction mixture (3.02 mL)

D: Enzyme dilution factor

6.2: mM extinction coefficient of NADPH (L･mmol-1･cm-1)

d: Light path length (1 cm)

v: Volume of enzyme sample (0.02 mL)

Reference Data

The product is intended for in vitro diagnostic or research use only.

Documents

INFO

SDS

Disclaimer: For laboratory research use only.

Glutamate Dehydrogenase (NADP+ )