Description

GFP-Expressing Rat Primary Epidermal Keratinocytes from Cell Biologics are isolated from the skin tissues of 6-8 weeks old laboratory Sprague–Dawley rat and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. GFP-Expressing Rat Primary Epidermal Keratinocytes are characterized by immunofluorescent staining with E-cadherin (sc-8426, Santa Cruz) or ZO-1 (MABT11, clone R40.76, Millipore) antibody. These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells are not recommended.

The green fluorescent protein (GFP) gives rise to very bright green fluorescence when exposed to blue light. Cell Biologics generates various GFP-expressing stable cells, including endothelial cells, epithelial cells and so forth. GFP-expressing cells are developed through transducing GFP-lentiviral particles.

Suggested Medium

M6621 Complete Epithelial Cell Medium w/ Kit – 500 ml

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

RA-6066K.GFP

Cell-Culture-Protocol-CB

1708462493

1698246847

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

GFP Expressing Rat Primary Epidermal Keratinocytes