Product Notice

Made to order product – estimated lead time: 1–2 months
Primary cell limitation: non-immortalized system with restricted expansion capacity
Passaging: not recommended by manufacturer; cells are intended for direct experimental use after recovery

Description

In blood-brain barrier (BBB) and neurovascular research, primary brain microvascular endothelial cells are essential for modeling endothelial transport and barrier integrity. However, conventional non-labeled primary cells often limit real-time visualization of dynamic processes such as endothelial migration, tight junction regulation, and permeability changes under physiological or disease conditions.

The GFP-expressing porcine primary brain microvascular endothelial cells (P-6023GFP) provide a physiologically relevant in vitro BBB model derived from porcine brain tissue. These primary endothelial cells are stably labeled via lentiviral GFP transduction, enabling bright green fluorescence for real-time tracking of cellular behavior during vascular remodeling and barrier function studies.

Functionality is confirmed by DiI-Ac-LDL uptake assay, demonstrating endothelial identity and metabolic activity. This model is widely used in blood-brain barrier research, neurovascular biology, CNS drug delivery studies, endothelial permeability assays, and live-cell imaging applications.

Features

Primary porcine brain microvascular endothelial cells with stable GFP expression
Physiologically relevant in vitro blood-brain barrier (BBB) model
Bright fluorescence enables real-time live cell imaging and tracking
Validated endothelial function via DiI-Ac-LDL uptake assay
Suitable for permeability, transport, and neurovascular studies
Limited passage primary system (3–5 passages recommended)
Compatible with gelatin-coated endothelial culture systems

Specifications

Cell Type	Porcine Brain Microvascular Endothelial Cells
Genetic Label	GFP (Green Fluorescent Protein), lentiviral stable expression
Functional Validation	DiI-Ac-LDL uptake assay
Model Type	Blood-Brain Barrier (BBB) in vitro model
Passage Range	3–5 passages recommended
Storage	Liquid nitrogen (-180°C)
Shipping	Dry ice (cryopreserved vial)
Catalog No.	P-6023GFP

Applications

Blood-brain barrier (BBB) permeability assays
CNS drug delivery and transport studies
Neurovascular biology and microcirculation research
Endothelial barrier integrity and tight junction analysis
Live-cell fluorescence imaging of endothelial dynamics
Angiogenesis and vascular remodeling studies
Drug screening for CNS-targeted therapeutics
Pathophysiological modeling of neurovascular disorders

GFP Fluorescent Imaging System

The integrated GFP labeling system enables high-resolution real-time visualization of endothelial cell behavior under blue-light excitation. This allows direct observation of:

Endothelial migration and alignment
Barrier integrity changes under stimulation
Microvascular network formation
Cell-cell interaction dynamics in BBB models

Cell Culture System

These primary endothelial cells are optimized for standard endothelial culture workflows using gelatin-coated flasks and endothelial growth medium systems.

Recommended coating: gelatin-based extracellular matrix
Culture system: endothelial growth medium (complete system)
Subculture ratio: 1:2 at ~90% confluence
Recommended handling: sterile biosafety conditions

Storage & Handling

Store cryovials in liquid nitrogen (-180°C) immediately upon arrival
Do not store at -80°C or -20°C
Avoid repeated freeze–thaw cycles
Use aseptic techniques during all handling steps
Protect GFP fluorescence from prolonged light exposure

Documents

P-6023GFP Datasheet

Cell Culture Protocol

Frequently Asked Questions

What is GFP-expressing porcine brain microvascular endothelial cells used for?

This cell model is primarily used for blood-brain barrier (BBB) research, CNS drug delivery studies, endothelial permeability assays, and neurovascular biology research.

Why use porcine brain microvascular endothelial cells for BBB models?

Porcine brain endothelial cells provide high physiological relevance due to similarities in brain microvascular structure and barrier function compared to human neurovascular systems.

What is the advantage of GFP labeling in this cell model?

GFP enables real-time fluorescence tracking of endothelial behavior, allowing researchers to observe migration, barrier changes, and vascular network formation without additional staining.

How is endothelial identity confirmed?

Endothelial phenotype is validated using DiI-Ac-LDL uptake assay, which confirms functional lipid uptake characteristic of endothelial cells.

What are the recommended storage conditions?

Cells must be stored in liquid nitrogen (-180°C). Storage at -80°C or -20°C is not recommended due to reduced viability and fluorescence stability.

Can these cells be used for permeability and drug transport studies?

Yes. These cells are widely used in BBB permeability assays and CNS drug transport studies to evaluate compound penetration across the blood-brain barrier.

GFP-Expressing Porcine Primary Brain Microvascular Endothelial Cells (0.5x10^6)