Product Notice

Made to order product – estimated lead time: 1–2 months
Primary cell limitation: limited expansion capacity typical of primary microvascular endothelial cells
Passaging: not recommended for long-term culture; use within early validated passages only

Description

In ovarian vascular biology and angiogenesis research, conventional endothelial cell lines often fail to replicate the complex microvascular environment required for studying follicular development, endocrine regulation, and reproductive tissue remodeling. This limitation restricts accurate modeling of ovarian microcirculation and vascular signaling pathways.

The GFP-expressing canine primary ovarian microvascular endothelial cells (D-6090GFP) provide a physiologically relevant primary endothelial model derived from beagle ovarian tissue. These cells maintain native microvascular characteristics and are stably labeled with green fluorescent protein (GFP), enabling real-time visualization of endothelial dynamics during angiogenesis and vascular remodeling.

Functional endothelial identity is confirmed by DiI-Ac-LDL uptake assay, supporting lipid uptake activity and endothelial phenotype stability. This model is widely used in ovarian angiogenesis research, reproductive vascular biology, endothelial permeability assays, and microcirculation studies.

Features

Primary canine ovarian microvascular endothelial cells with stable GFP expression
Physiologically relevant ovarian angiogenesis model
Real-time fluorescence imaging for vascular dynamics tracking
Validated endothelial function via DiI-Ac-LDL uptake assay
Suitable for angiogenesis, permeability, and vascular remodeling studies
Limited passage primary endothelial system
Compatible with gelatin-coated endothelial culture conditions

Specifications

Cell Type	Canine Ovarian Microvascular Endothelial Cells
Species	Beagle Dog
Genetic Label	GFP (Green Fluorescent Protein), lentiviral expression
Functional Validation	DiI-Ac-LDL uptake assay
Model Type	Ovarian microvascular / angiogenesis model
Storage	Liquid nitrogen (-180°C)
Shipping	Dry ice (cryopreserved vial)
Catalog No.	D-6090GFP

Applications

Ovarian angiogenesis research models
Reproductive vascular biology studies
Endothelial permeability and barrier function assays
Follicular microcirculation research
Drug screening for reproductive system targeting
Hormonal regulation of vascular networks
Inflammation-related vascular response studies
Live-cell imaging of endothelial migration and remodeling

Ovarian Angiogenesis & Microvascular System

This GFP-labeled endothelial model enables real-time analysis of ovarian vascular remodeling and angiogenic signaling. It is particularly suitable for:

Endothelial sprouting and vessel formation studies
Microvascular permeability assays
Follicle-associated vascular development
Reproductive tissue vascular interaction studies

Cell Culture System

Recommended coating: gelatin-based extracellular matrix
Endothelial growth medium compatible system
Supports short-term primary culture workflows
Recommended early-passage experimental use

Storage & Handling

Store in liquid nitrogen (-180°C) immediately upon arrival
Do not store at -80°C or -20°C
Avoid repeated freeze–thaw cycles
Use sterile biosafety techniques
Protect GFP fluorescence from prolonged light exposure

Documents

D-6090GFP Datasheet

Cell Culture Protocol

Frequently Asked Questions

What are GFP-expressing canine ovarian microvascular endothelial cells used for?

They are used for ovarian angiogenesis research, reproductive vascular biology, endothelial permeability assays, and microcirculation studies.

Why are ovarian microvascular endothelial cells important?

They regulate follicular development, hormonal transport, and vascular remodeling in the ovarian microenvironment.

What is the benefit of GFP labeling in this model?

GFP enables real-time visualization of endothelial migration, angiogenesis, and vascular remodeling without additional staining.

How is endothelial identity validated?

Endothelial phenotype is confirmed using DiI-Ac-LDL uptake assay, indicating functional lipid uptake activity.

What research fields use this model?

It is widely used in angiogenesis research, reproductive biology, vascular permeability studies, and drug screening.

Can this model be used for drug screening?

Yes, it is suitable for screening compounds targeting ovarian vascular function and angiogenic pathways.

GFP-Expressing Canine Primary Ovarian Microvascular Endothelial Cells (0.5x10^6)