Product Notice

Made to order product – estimated lead time: 1–2 months
Primary cell limitation: limited expansion capacity typical of primary alveolar epithelial cells
Passaging: not recommended for long-term culture; use within validated early passages

Description

In respiratory and pulmonary research, traditional epithelial cell lines often fail to reproduce the physiological complexity of alveolar barrier function, especially in studies involving air-liquid interface (ALI) culture, inhalation toxicity, and lung injury modeling. This limitation makes it difficult to accurately simulate in vivo lung epithelial responses.

The GFP-expressing canine primary alveolar epithelial cells (D-6053GFP) provide a physiologically relevant primary lung epithelial model derived from beagle alveolar tissue. These cells retain native epithelial characteristics and are stably labeled with green fluorescent protein (GFP), enabling real-time visualization of cellular dynamics in lung barrier systems.

This model is validated by ZO-1 tight junction expression, confirming epithelial barrier integrity. It is widely used in alveolar epithelial barrier research, ALI (air-liquid interface) models, pulmonary fibrosis studies, ARDS research, and inhalation toxicity screening.

Features

Primary canine alveolar epithelial cells with stable GFP expression
Physiologically relevant lung epithelial barrier model
Suitable for air-liquid interface (ALI) culture systems
Validated by ZO-1 tight junction protein expression
Supports live cell imaging and fluorescent tracking
Limited passage primary cell system
Compatible with standard epithelial culture conditions

Specifications

Cell Type	Canine Primary Alveolar Epithelial Cells
Species	Beagle Dog
Genetic Label	GFP (Green Fluorescent Protein), lentiviral expression
Functional Validation	ZO-1 tight junction immunofluorescence
Model Type	Lung epithelial barrier / ALI model
Storage	Liquid nitrogen (-180°C)
Shipping	Dry ice (cryopreserved vial)
Catalog No.	D-6053GFP

Applications

Air-liquid interface (ALI) lung epithelial modeling
Pulmonary fibrosis mechanism research
Acute lung injury (ALI/ARDS) in vitro models
Inhalation toxicity and nanomaterial exposure studies
Lung epithelial barrier integrity assays
Drug screening for respiratory system therapeutics
Airway inflammation and epithelial repair studies
Live-cell imaging of alveolar epithelial dynamics

Alveolar Epithelial Barrier System

This GFP-labeled model enables real-time visualization of alveolar epithelial behavior under physiological or injury conditions. It is particularly suitable for:

Air-liquid interface (ALI) differentiation studies
Tight junction formation and disruption analysis
Epithelial wound repair and regeneration
Barrier permeability and transport studies

Cell Culture System

Recommended coating: gelatin-based extracellular matrix
Suitable for epithelial growth medium systems
Supports ALI culture adaptation workflows
Recommended for early-passage experimental use

Storage & Handling

Store in liquid nitrogen (-180°C) immediately upon arrival
Do not store at -80°C or -20°C
Avoid repeated freeze–thaw cycles
Use sterile biosafety techniques during handling
Minimize light exposure to preserve GFP fluorescence

Documents

D-6053GFP Datasheet

Cell Culture Protocol

Frequently Asked Questions

What are GFP-expressing canine primary alveolar epithelial cells used for?

They are used for lung epithelial barrier research, ALI (air-liquid interface) models, pulmonary fibrosis studies, and inhalation toxicity testing.

Why are alveolar epithelial cells important in lung research?

Alveolar epithelial cells form the primary barrier for gas exchange and are essential for studying lung injury, inflammation, and respiratory disease mechanisms.

What is the advantage of GFP labeling in this model?

GFP enables real-time visualization of epithelial cell behavior, including migration, barrier formation, and injury response without additional staining.

Can this model be used for ALI (air-liquid interface) studies?

Yes, this model is compatible with ALI systems and is widely used for differentiation and epithelial barrier function studies.

How is epithelial integrity validated?

Epithelial phenotype is confirmed by ZO-1 tight junction expression, indicating functional barrier formation.

What diseases can this model simulate?

It is widely used in pulmonary fibrosis, acute lung injury (ALI/ARDS), airway inflammation, and inhalation toxicity research.

GFP-Expressing Canine Primary Alveolar Epithelial Cells (0.5x10^6)