Description

GFP-Expressing Mouse Primary Colonic Endothelial Cells from Cell Biologics are isolated from colonic tissue of pathogen-free laboratory C57BL/6 mice and grown in gelatin pre-coated tissue culture flask with Cell Biologics’ Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo-preserved in vials. Each vial contains 0.5x10⁶ cells per ml and is delivered frozen. GFP-Expressing Mouse Primary Colonic Endothelial Cells are characterized by immunofluorescence staining with antibodies of VE-cadherin (CD144, VE-cadherin Antibody, C-19, sc6458, Santa Cruz), AF1002 (R&D System) or CD31/PECAM-1 (Catalog No. 553370, BD). These cells are negative for bacteria, yeast, fungi, and mycoplasma and can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Cell Biologics. Repeated freezing and thawing of cells is not recommended.

The green fluorescent protein (GFP) gives rise to very bright green fluorescence when exposed to blue light. Cell Biologics generates various GFP-expressing stable cells, including Macrophages, epithelial cells and so forth. GFP-expressing cells are developed through transducing GFP-lentiviral particles.

Suggested Medium

M1168 Complete Endothelial Cell Medium w/ Kit – 500 ml

Storage

Cryopreserved cells are shipped with dry ice overnight. Upon arrival, transfer frozen cells to liquid nitrogen (-180°C) immediately until ready for use. Live cell shipment is also available on request. Primary cells can never be kept at -20 °C or -80 °C freezer.

Documents

C57-6203GFP

Cell-Culture-Protocol-CB

1708462493

1698246847

Disclaimer

Investigators should handle the cells with caution and treat all animal cells as potential pathogens, since no test procedure can completely guarantee the absence of infectious agents.

GFP Expressing C57BL/6 Mouse Primary Colonic Microvascular Endothelial Cells