Name: Gentamicin Sulfate 50 mg/mL Solution
Brand: Biofargo

Description

In routine cell culture and tissue culture workflows, bacterial contamination remains one of the most common causes of experimental failure, leading to compromised cell viability, inconsistent results, and loss of valuable samples. Reliable contamination control is therefore essential for maintaining stable and reproducible in vitro systems.

:contentReference[oaicite:0]{index=0} is a broad-spectrum aminoglycoside antibiotic widely used in laboratory research to prevent or eliminate bacterial contamination in mammalian cell culture systems. It is effective against both Gram-negative and Gram-positive bacteria and functions by binding to the 30S ribosomal subunit, thereby inhibiting bacterial protein synthesis and preventing microbial growth.

This sterile Gentamicin Sulfate solution is supplied at 50 mg/mL concentration, providing a ready-to-use contamination control reagent for cell culture media supplementation, ensuring stable and reproducible experimental conditions in molecular biology, biotechnology, and cell-based assays.

Specification

Grade	USP
Appearance	Liquid
Concentration	50 mg/mL
Sterility	Sterile-filtered solution
Storage	2–8°C, protect from light, warm to room temperature before use
Application Grade	Cell Culture / Molecular Biology Grade
Use	Research Use Only (RUO)

Features

Broad-spectrum cell culture antibiotic for effective bacterial contamination control in mammalian and primary cell culture systems.
Mechanism-based inhibition of bacterial protein synthesis via 30S ribosomal subunit binding.
Sterile ready-to-use liquid formulation (50 mg/mL) for convenient media supplementation.
Effective against Gram-negative and Gram-positive bacteria in routine laboratory environments.
USP-grade quality for consistent performance in cell culture and molecular biology workflows.
Compatible with standard cell culture media including DMEM, RPMI-1640, and serum-containing systems.

Applications

Cell culture contamination control in mammalian and primary cell systems.
Tissue culture maintenance and routine cell line propagation.
Molecular biology and biotechnology research workflows requiring sterile conditions.
Cell-based assays including proliferation, cytotoxicity, and signaling studies.
Laboratory media supplementation for bacterial suppression during long-term culture.
Biopharmaceutical R&D and preclinical research applications.

Mechanism of Action

Gentamicin Sulfate belongs to the aminoglycoside class of antibiotics. It exerts its antibacterial effect by binding irreversibly to the bacterial 30S ribosomal subunit, leading to misreading of mRNA and inhibition of protein synthesis. This results in effective bactericidal activity and suppression of microbial contamination in cell culture environments.

Documents

COA SDS

Frequently Asked Questions

What is Gentamicin Sulfate used for in cell culture?

It is used as a broad-spectrum cell culture antibiotic to prevent or eliminate bacterial contamination in mammalian and tissue culture systems.

How does Gentamicin work against bacteria?

It binds to the bacterial 30S ribosomal subunit, causing inhibition of protein synthesis and leading to bactericidal activity.

What is the typical working concentration in cell culture?

It is commonly used in the range of 10–50 µg/mL after dilution from the 50 mg/mL stock solution.

Is Gentamicin effective against both Gram-negative and Gram-positive bacteria?

Yes, it shows broad-spectrum activity, with stronger efficacy against Gram-negative bacteria.

Can it replace Penicillin-Streptomycin (Pen/Strep)?

It can be used as an alternative in some workflows, especially when specific antibiotic profiles or resistance considerations are required.

How should it be stored?

Store at 2–8°C and avoid repeated freeze-thaw cycles to maintain stability.

For laboratory research or industrial use only.

Gentamycin Sulfate, 50MG/ml Solution