Description

This kit uses a DNA-Only Column that can specifically bind DNA, a brand-new Foregene Protease and a unique buffer system, which can efficiently extract various trace biological samples, such as trace amounts of blood, cells, and animals within 20-80 minutes. Tissues, dried bloodstains, clinical cotton swabs, hair follicles, etc., are purified to obtain high-concentration, high-quality genomic DNA.

The DNA-Only silica gel membrane used in the spin column is a unique new material of our company, which efficiently and specifically adsorbs DNA, and can remove RNA, impurity proteins, ions and other organic compounds in cells to the greatest extent. The specially designed mini-purification column binds genomic DNA, and the DNA can be eluted with a small amount (15μl) of eluent to increase the concentration of the obtained genomic DNA, which is convenient for downstream detection or experiment.

Features

No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove the RNA from the genomic DNA without adding RNase during the experiment, preventing the laboratory from being contaminated by exogenous RNase.
Fast speed: Foregene Protease has higher activity than similar proteases, and digests tissue samples quickly; the operation is simple, and the genomic DNA extraction operation can be completed within 20-80 minutes.
Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.
Safety: No organic reagent extraction is required.
High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.
Micro-elution system: It can increase the concentration of genomic DNA, which is convenient for downstream detection or experiment.

Application

It is suitable for the purification of the following samples of genomic DNA: fresh or frozen animal tissues, fresh or anticoagulated cryopreserved trace amounts of whole blood, dried blood spots, cultured cells, microsection tissues, hair follicles, mouthwashes, etc.

Work Flow

Storage

This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
Foregene Protease solution has a unique formula, which is active when stored at room temperature for a long time (3 months); its activity and stability will be better when stored at 4°C, so it is recommended to store at 4°C, remember not to keep it at -20°C .

Documents

Genomic DNA Micro Kit Instruction Manual-V1.0-1403-EN