{"product_id":"gennext-ramda-seq-single-cell-kit","title":"GenNext RamDA-seq Single Cell Kit-Toyobo","description":"\u003cdiv style=\"font-family: 'Helvetica Neue', Helvetica, Arial, sans-serif; color: #333; line-height: 1.6; max-width: 1200px; margin: 0 auto; padding: 10px;\"\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDescription\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eGenNext™ RamDA-seq™ Single Cell Kit is a kit using RT-RamDA™ (Reverse Transcription with Random Displacement Amplification) method for preparing cDNA for NGS analysis from single-cell and Ultra-low amount RNA.\u003c\/p\u003e\n\u003cp\u003eRT-RamDA™ is a novel cDNA amplification method utilizing the strand displacement activity of reverse-transcriptase. GenNext RamDA-seq™ Single Cell Kit can detect not only poly(A) RNA but also non-poly(A) with high sensitively by using random primer or NSR primer*, allowing the detection of many genes compared with the conventional method.\u003cbr\u003eUnlike conventional method using Oligo-dT primer, cDNA for full-length total RNA-seq can be synthesized by RT-RamDA™ method.\u003c\/p\u003e\n\u003cp\u003eIn addition, because cDNA is amplified at the same time as it is synthesized, RamDA-seq™ method does not require amplification adaptors and PCR amplification step, and it reduces the biases caused by PCR amplification.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_ngs002_01.png?v=1775547097\" alt=\"\"\u003e\u003c\/div\u003e\n\u003cp\u003e* NSR primer\u003cbr\u003eNSR primer is abbreviation for Not so random primer. NSR primer is designed random primer to avoid synthesizing cDNA from the rRNAs by removing 6-mers that exactly match the rRNA sequences from N6 random primers.\u003cbr\u003eIn order to perform more efficient NGS analysis, it is necessary to reduce the rRNA contamination rate.As a countermeasure, we also offer Not so random primers for humans and mice sold separately.\u003c\/p\u003e\n\u003cp style=\"margin-bottom: 0;\"\u003e* RamDA-seq™ and RT-RamDA™ are a trademark of RIKEN, Institute of Physical and Chemical Research\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eFeatures\u003c\/h2\u003e\n\u003cul style=\"list-style-type: none; padding-left: 0;\"\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e cDNA can be prepared from a single cell or small amount of input RNA\u003cbr\u003e1-100 cells or 10 pg-1 ng total RNA\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Full length cDNA can be analyzed\u003cbr\u003ecDNA that covers the entire length of the target RNA of 10 kb or more can be prepared.\u003c\/li\u003e\n\u003cli style=\"margin-bottom: 15px; padding-left: 25px; position: relative;\"\u003e\n\u003cspan style=\"position: absolute; left: 0; color: #003366;\"\u003e•\u003c\/span\u003e Various RNAs can be detected, and the number of genes detect able is higher than that with conventional technology.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cdiv style=\"padding-left: 25px;\"\u003e\n\u003cp style=\"margin: 0 0 8px 0;\"\u003e- Identification of isoforms and alternative splicing\u003c\/p\u003e\n\u003cp style=\"margin: 0 0 8px 0;\"\u003e- Detection of poly(A) RNA and non poly(A) RNA (histone RNA and lncRNA)\u003c\/p\u003e\n\u003cp style=\"margin: 0;\"\u003e- Detection of nuclear RNA (pre mRNA and lncRNA)\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eSpecification\u003c\/h2\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eCategory\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eItem\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eDetails\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; font-weight: bold; background-color: #fcfcfc;\"\u003eStorage condution\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: center;\"\u003e-\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eStore at-20 ℃\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eComponents\u003c\/h2\u003e\n\u003cp\u003eThe kits include the following reagents that can be used for 96 (RMD-101) and 24 (RMD-101T) reactions. All reagents should be stored at −20℃.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 900px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eGenNext™ RamDA-seq™ Single Cell Kits\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eRMD-101\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eRMD-101T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eSize\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e96 Rxns\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e24 Rxns\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eLysis Buffer\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e480 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e120 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eLysis Enhancer\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e108 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e27 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRNase Inhibitor\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e22 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e6 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eNuclease free water\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e960 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e240 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRT-RamDA™ Buffer\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e240 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e60 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRT-RamDA™ Enzyme Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e54 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e14 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003eRT-RamDA™ Primer Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e54 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e14 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003egDNA Remover\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e54 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e14 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e2nd strand synthesis Buffer\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e330 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e83 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e2nd strand synthesis Enzyme\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e55 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e14 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e2nd strand synthesis Primer Mix\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e275 μL\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e69 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cp\u003e*Do not store mixed solution.\u003c\/p\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eNSR Primer Set for human\u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eNSR-101\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1st NSR Primer Mix for human\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e54 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e2nd NSR Primer Mix for human\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e275 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"overflow-x: auto; margin-bottom: 20px;\"\u003e\n\u003ctable style=\"width: 100%; border-collapse: collapse; border: 1px solid #e0e0e0; min-width: 600px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"background-color: #003366; color: #ffffff;\"\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eNSR Primer Set for mouse \u003c\/th\u003e\n\u003cth style=\"padding: 12px; border: 1px solid #e0e0e0; text-align: left;\"\u003eNSR-102\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e1st NSR Primer Mix for mouse\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e54 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"background-color: #fafafa;\"\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e2nd NSR Primer Mix for mouse\u003c\/td\u003e\n\u003ctd style=\"padding: 12px; border: 1px solid #e0e0e0;\"\u003e275 μL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eRequired materials not included\u003c\/h2\u003e\n\u003cul style=\"padding-left: 20px; margin-bottom: 0;\"\u003e\n\u003cli\u003eThermocycler\u003c\/li\u003e\n\u003cli\u003eLibrary preparation reagents\u003cbr\u003eGenNext RamDA-seq™ Single Cell Kits are specifically for use with the Illumina Nextera™ XT DNA Sample Kit.\u003c\/li\u003e\n\u003cli\u003eNextera™ XT DNA Sample Kit (24 Samples), Cat. no. FC-131-1024\u003c\/li\u003e\n\u003cli\u003eNextera™ XT DNA Sample Kit (96 Samples), Cat. no. FC-131-1096\u003c\/li\u003e\n\u003cli\u003eSPRI (Solid Phase Reversible Immobilization) paramagnetic beads\u003c\/li\u003e\n\u003cli\u003eAgencourt™ AMPure™ XP Beads (Beckman Coulter, Cat. no. A63880 or A63881)\u003c\/li\u003e\n\u003cli\u003eTE Buffer pH 8.0 (10 mM Tris-HCl,,1mM EDTA)\u003c\/li\u003e\n\u003cli\u003eMagnetic rack\/stand for magnetic bead separation\u003c\/li\u003e\n\u003cli\u003e80% Ethanol (freshly prepared)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003ePrinciple of RT-RamDA™ method\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cp style=\"margin-top: 0;\"\u003eRT-RamDA™ is an abbreviation for \"Reverse Transcription with Random Displacement Amplification\" developed by the Bioinformatics Research and Development Team, RIKEN Center for Biosystems Science and Technology.It is a new cDNA amplification method that applies the strand substitution activity of reverse transcriptase, and it is possible to prepare cDNA not only from poly (A) RNA but also from non-poly (A) -derived RNA for using raondom primer.\u003cbr\u003eUnlike conventional method using Oligo-dT primer, cDNA for full-length total RNA-seq can be synthesized by RT-RamDA™ method.\u003cbr\u003eIn addition, because cDNA is amplified at the same time as it is synthesized, RT-RamDA™ method does not require amplification adaptors and PCR amplification step, and it reduces the biases caused by PCR amplification.\u003cbr\u003e* Hayashi .T et al. Single-cell full-length total RNA sequencing uncovers dynamics of recursive splicing and enhancer RNAs. Nature Communications. 9：619（2018)\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0 0 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_ngs002_02.png?v=1775547346\" alt=\"\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eWorkflow\u003c\/h2\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RMD_Workflow2.jpg?v=1775547346\" alt=\"\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eApplication Data\u003c\/h2\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 1.Comparison of the number of detected transcripts and the alignment ratio within each region\u003c\/h3\u003e\n\u003cp\u003eUsing next generation sequencing data, cDNA and double-stranded DNA were prepared using either the GenNext™ RamDA-seq™ Single Cell Kit (Code No. RMD-101) or a Company A kit, with a sample input of 10 pg of total RNA extracted from mouse ES cells. The GenNext™ RamDA-seq™ Single Cell Kit uses the NSR Primer Set for mouse (code No.NSR-102). PCR for cDNA amplification, which is not required with the GenNext™ RamDA-seq™ Single Cell Kit, was performed for 18 cycles with the Company A kit. The libraries were then prepared with the Nextera™ XT DNA Library Preparation Kit and sequenced with the Illumina MiSeq instrument. Thus, the GenNext™ RamDA-seq™ Single Cell Kit was shown to detect approximately 5,000 more genes than the Company A kit.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_ngs002_03.png?v=1775547510\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 2.Comparison of the full-length coverage of long RNA molecules\u003c\/h3\u003e\n\u003cp\u003eAnalysis was performed on the sequence data obtained with the method described for Example 1. The GenNext™ RamDA-seq™ Single Cell Kit can detect lncRNAs such as Neat1 and Malat1, which were difficult to capture with the Company A kit over their entire length.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/img_ngs002_04.png?v=1775547510\" alt=\"\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 3.Comparison of the rate of rRNA contamination using random Primers or NSR Primers\u003c\/h3\u003e\n\u003cp\u003eUsing the RT-RamDA™ Primer Mix(random Primers) included with the GenNext™ RamDA-seq™ Single Cell Kit [Code No. RMD-101] or the separately sold NSR Primer Set for mouse [Code No. NSR-102], cDNA and double-stranded DNA were synthesized from total RNA (10pg, 1ng) extracted from NIH3T3 cells. libraries were prepared using the Nextera™ XT DNA Library Preparation Kit and Sequencing was performed on an Illumina™ MiSeq™ instrument.\u003cbr\u003eFor both 10pg and 1ng of Total RNA, NSR Primers resulted in the lower rate of rRNA contamination compared to random primers.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/Applocation_3.png?v=1775547510\"\u003e\u003c\/div\u003e\n\u003cp\u003eUsing NSR primers can reduce the rate of rRNA contamination more than random primers.\u003cbr\u003eFor human-derived samples, it is recommended to use the NSR Primer Set for human [Code No. NSR-101], and for mouse-derived samples, the NSR Primer Set for mouse [Code No. NSR-102]\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 40px; border: 1px solid #eee; padding: 20px; border-radius: 8px;\"\u003e\n\u003ch3 style=\"color: #333; font-size: 18px; margin-top: 0;\"\u003eExample 4.RNA-seq analysis from various RNA input amounts (2 pg to 6.25 ng)\u003c\/h3\u003e\n\u003cp\u003eUsing RNA extracted from HeLa cells, libraries were prepared with input amounts ranging from 2 pg to 6.25 ng. For cDNA synthesis, the NSR Primer Set for human [Code No. NSR-101] was used, and all library enrichments were performed with 15 cycles.Sequencing was performed on an Illumina™ MiSeq™ instrument, and NGS analysis was performed using basespace (Illumina) RNA-Seq Alignment (ver. 2.0.2) or RamDAQ (ver.1.9).\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/application4.png?v=1775547510\"\u003e\u003c\/div\u003e\n\u003cp\u003eThe number of detected transcripts and genes was low at 2 pg because the input amount was low to begin with. The number of detected transcripts and genes was high at input amounts other than 2 pg, and the coverage was unbiased at all input amounts.\u003c\/p\u003e\n\u003cdiv style=\"text-align: center; margin: 20px 0;\"\u003e\u003cimg style=\"max-width: 100%; height: auto;\" alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/application_42.png?v=1775547510\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003ePublications Using GenNext™ RamDA-seq™ Single Cell Kit\u003c\/h2\u003e\n\u003cdiv style=\"background-color: #f9f9f9; padding: 20px; border-radius: 4px; border: 1px solid #eee;\"\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cp\u003eYoshimatsu, S. et al., Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts of Multiple Mammalian Species. Stem Cell Reports, Volume 16:754-770(2021)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eHasegawa, N. et al., Highly sensitive fusion detection using plasma cell-free RNA in non-small-cell lung cancers. Cancer Sci. 112(10):4393-4403(2021)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eOka, Y. et al., A novel sorting signal for RNA packaging into small extracellular vesicles. Scientific Reports 13, Article number: 17436 (2023)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003cli\u003e\n\u003cp\u003eUsuda, M. et al., Inhibitory Effect of Adsorption of Streptococcus mutans onto Scallop-Derived Hydroxyapatite. Int. J. Mol. Sci. 24,11371 (2023)\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv style=\"margin-bottom: 30px;\"\u003e\n\u003ch2 style=\"color: #003366;  padding-left: 15px; font-size: 24px; margin-bottom: 20px; font-weight: bold;\"\u003eDocuments\u003c\/h2\u003e\n  \u003cdiv style=\"border: 1px solid #eee; background-color: #f9f9f9; padding: 24px; border-radius: 8px;\"\u003e\n\u003cdiv style=\"display: flex; flex-wrap: wrap; gap: 15px;\"\u003e\n\u003ca style=\"display: inline-block; background-color: #003366; color: #ffffff; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RMD-101_manual_EN.pdf?v=1775806977\" target=\"_blank\"\u003e Download Manual \u003c\/a\u003e \n\n  \u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/RMD-101_SDS.pdf?v=1775806972\" target=\"_blank\"\u003e Download SDS \u003c\/a\u003e\n\n\n\u003ca style=\"display: inline-block; background-color: #ffffff; color: #003366; text-decoration: none; padding: 12px 24px; border-radius: 4px; font-size: 15px; font-weight: bold; border: 1px solid #003366;\" rel=\"noopener noreferrer\" href=\"https:\/\/biofargo.com\/pages\/contact-us?srsltid=AfmBOorrYySVXlrC6mOen85FBiTKooBmPEyrEjjsqXyC0kKPRm7fDFPV\" target=\"_blank\"\u003e Request COA \u003c\/a\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n","brand":"Toyobo","offers":[{"title":"24Rxn","offer_id":52417796079797,"sku":"RMD-101T","price":1144.8,"currency_code":"USD","in_stock":false},{"title":"96Rxn","offer_id":52417796112565,"sku":"RMD-101","price":3816.0,"currency_code":"USD","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0521\/5312\/2997\/files\/GenNext_RamDA-seq_Single_Cell_Kit-Toyobo.jpg?v=1777517603","url":"https:\/\/biofargo.com\/products\/gennext-ramda-seq-single-cell-kit","provider":"Biofargo","version":"1.0","type":"link"}