Descriptions

Quickly purify high-quality plasmid DNA from transformed bacteria for routine molecular biology experiments such as transformation and enzyme digestion

Features

Remove RNA without adding RNase
Convenient—the centrifugation is performed at room temperature, 4℃ centrifugation and ethanol precipitation of DNA is not required.
Fast—the operation can be completed in 20 minutes
Safe—no organic reagent used
High purity—OD260/280≈1.7-1.9

Application

It is suitable for the extraction and purification of total RNA from fresh or frozen plant tissue samples (especially fresh plant leaf tissue) with low polysaccharide and polyphenol content.

Kit parameters

No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove the RNA in the plasmid DNA without adding RNase during the experiment, avoiding the contamination of the laboratory by exogenous RNase.
Fast speed: Plasmid DNA extraction can be completed within 20 minutes.
Safe: No organic reagent extraction is required.
High quality: The plasmid obtained by extraction has no RNA, no RNase and extremely low ion content, which can meet the requirements of various experiments.

Work Flow

Diagram:

Storage

This kit can be stored for 12 months under dry conditions at room temperature (15-25℃). If it needs to be stored for a longer time, it can be stored in 2-8℃.

Document

General Plasmid Mini Kit Instruction Manual-V2.0-2008-EN