Description

The Garlic Common Latent Virus (GCLV) ELISA Kit is designed for the sensitive and specific detection of Garlic Common Latent Virus (GCLV) in infected garlic plants. This enzyme-linked immunosorbent assay (ELISA) provides a reliable serological method for identifying GCLV in plant tissue samples and is suitable for routine screening and research applications.

Garlic common latent virus is the most prevalent garlic carlavirus and has been reported worldwide, including Asia, Europe, South America, and North America. In addition to garlic, GCLV is known to infect leek, onion, and chives. This kit enables accurate detection while minimizing cross-reactivity with related plant viruses.

This product is intended for research use only and is not intended for diagnostic or therapeutic use.

Target Information

Targe	Garlic Common Latent Virus (GCLV)
Virus Family	Betaflexiviridae
Genus	Carlavirus
Alternative Name	Previously described as garlic latent virus (renamed to avoid confusion with Japanese GLV)
Host Species	Garlic (Allium sativum), leek, onion, chives

Assay Principle

This kit is based on a solid-phase enzyme-linked immunosorbent assay (ELISA). Specific antibodies are used to coat a high-binding microtiter plate, capturing GCLV antigens present in plant extracts. An enzyme-labeled antibody conjugate is then applied for detection.

Upon addition of the chromogenic substrate, a color reaction develops in positive samples. Results can be interpreted visually or quantified using a microplate reader at 405 nm.

Kit Components

Each kit contains all reagents required to perform the ELISA assay:
Capture antibody specific for GCLV
Enzyme-conjugated detecting antibody
High-binding ELISA microtiter plate
Positive control (lyophilized)
Negative control (lyophilized)
Coating buffer
Sample buffer
Enzyme conjugate buffer
Washing buffer
Substrate buffer and PNP tablets
Instruction manual

Sample Types

Leaf tissue (recommended)
Stem
Sprout
Seed
Bulb
Root

Plant tissues can be extracted using the provided sample buffer at a recommended ratio of 1:10 (tissue or sap to buffer).

Performance Characteristics

Sensitivity

The sensitivity of the ELISA assay is moderately high. GCLV can be consistently detected in infected plant tissues diluted up to 1:270.

Specificity

The assay reacts with all tested isolates of GCLV and produces a strong signal. Optical density values at 405 nm typically range from 1.480 to 3.000, depending on virus titer.

No cross-reactivity was observed with other carlaviruses, including CLV, BBScV, KLV, LSV, PVM, PVS, and SLV. A weak cross-reaction was observed with CVB. No cross-reaction was detected with healthy garlic plant tissues, and background signal in negative controls is low.

Storage and Stability

All kit components should be stored at the recommended temperature to ensure stability and performance. When stored properly, the kit should be used within six months of purchase.

Documents

GCLV-MSDS

GCLV-Data Sheet

Regulatory Status

For Research Use Only (RUO).

Not for diagnostic, therapeutic, or clinical use.