Description

Biofargo™ Precast Protein Gels are polyacrylamide electrophoresis gels designed for the separation of a wide range of protein sizes. They are available in 12-well and 15-well formats, with large sample loading capacities—up to 50 µL per well for the 12-well format and 30 µL per well for the 15-well format.

Produced using automated gel-casting technology, Biofargo™ gels ensure excellent batch-to-batch consistency and superior quality. The unique gel buffer formula delivers sharper bands and higher resolution, while the neutral pH prevents protein re-modification during electrophoresis, thereby improving protein stability and gel performance.

Compared with traditional hand-cast gels, Biofargo™ Precast Protein Gels offer the following advantages:

Easy to use: Ready-to-use format saves preparation time.
Safe and reliable: Eliminates the need to handle toxic reagents.
Accurate and reproducible results: Automated casting technology ensures consistent quality and experimental reproducibility.

Operation Procedure

2.1 Take the Biofargo™ precast protein gels out of the package and remove the tape at the bottom of the gel plate (see Figure 1).

Figure1.Removethetapeatthebottomofthegelplate.

2.2 Push the comb smoothly out of the gel plate in the direction of the arrow (see Figure 2).

Figure2.Removethecombfromthegelplate.

2.3 Insert the gel plate into the gel electrophoresis apparatus (see Figure 3).

Figure3.Insertthegelplateintothegelelectrophoresisapparatus.

2.4 Complete the installation according to the apparatus manufacturer’s instructions (see Figure 4). Pour enough appropriate buffer into the inner tank of the apparatus to cover the wells by 5–7 mm. Add the same buffer to the outer tank to ensure proper cooling. For best results, the buffer in the outer tank should be slightly lower than that in the inner tank and must not cover the gel plates.

Figure4.Completealltheinstallation.

2.5 Use a syringe or other suitable tools to rinse the wells with 1× electrophoretic buffer and remove bubbles and residual storage buffer. Load the protein samples into the wells and start electrophoresis.

2.6 Remove the gel from the plate (see Figure 5):

Figure5.Openthegelcassettewithanopener.

Once electrophoresis is finished, remove the gel plate from the apparatus.
Open the gel cassette carefully by inserting the opener into the gap between the two plates.
Wiggle the opener gently up and down, repeating the operation until the two plates are completely separated.
Upon opening, the gel may remain on either side of the cassette. Discard the plate without the gel, and loosen the gel from the other plate with water, then gently remove it.

2.7 Perform staining or protein transfer according to the experimental requirements.

Data Display

Voltage: 160 V

Running Buffer: MOPS Running Buffer (50 mM Tris, 50 mM MOPS, 0.1% SDS, 1 mM EDTA, pH 7.7)

Staining Method: Coomassie staining

Gel Separation Range

Different concentrations of Biofargo™ precast protein gels have different separation ranges. Detailed data are shown in Figure 6.

Figure 6. The protein separation range of the different gels.

Storage

4 °C

FuturePAGE 4-12% 12 Wells-X