Description

This kit adopts a spin column with high affinity for DNA, together with a unique buffer system, to allow rapid extraction of fungal genomic DNA. It is particularly suitable for extracting genomic DNA from molds (e.g., Aspergillus niger, Trichoderma, Penicillium), yeasts, and mushrooms (e.g., Lentinula edodes, Flammulina velutipes). The method ensures high yield and excellent stability.

The DNA recovered using this kit is suitable for downstream applications such as restriction digestion, PCR, and library prep.

Features

Broad applicability: Suitable for the extraction of genomic DNA from molds (Aspergillus niger, Trichoderma, Penicillium), yeasts, and mushrooms (Lentinula edodes, Flammulina velutipes). It can also be used to extract genomic DNA from certain bacteria (e.g., Lactobacillus, Escherichia coli).
Ease of use: The unique buffer system eliminates the need for Proteinase K or RNase A digestion, preventing protein and RNA contamination, and enables completion of the procedure in a relatively short time.
High purity: The purified DNA can be directly used in molecular biology experiments such as PCR, restriction digestion, and hybridization.

User-Supplied Reagents and Equipment

Absolute ethanol
Isopropanol
Liquid nitrogen or a tissue homogenizer (TGrinder H24 Tissue Homogenizer, TIANGEN, Cat. No. OSE-TH-01, recommended)

Fungal Genomic DNA Extraction Kit