Description

FREY agar is fortified with nicotinamide adenine dinucleotide (NAD⁺) to satisfy the fastidious growth requirements of Mycoplasma synoviae. Metabolic activity of viable mycoplasmas produces acidic by-products that trigger a phenol red pH indicator shift from red to yellow—or, under alkaline drift, to purple—thereby providing an immediate visual confirmation of proliferation. The inclusion of penicillin G selectively suppresses contaminating bacterial flora without impairing mycoplasma growth.

In addition, the medium can be further enriched with a defined lipid supplement from yolk, supplying bioavailable cholesterol and fatty-acid precursors, markedly enhancing colony-forming efficiency—particularly for lipid-dependent strains such as Mycoplasma pneumoniae ATCC 23714.

Specifications

Agency	EP 2.6.7 / USP / JP
Quality Level	ISO 9001
Sterility	Sterile
-	Aseptically Filled
Form	Liquid
Feature	Ready-to-use; ICR Settler plates
Packaging	1 bottle
Bottle Filling Volume	100 mL
pH	7.8 (in H2O)
Application(s)	Industrial qc
Storage Temp	2-8℃ for 3 months; -20℃ to -80℃ for 6 months
Suitability	Mycoplasma

Application

FREY agar is recommended for the detection of avian mycoplasmas in pharmaceutical products, particularly in bulk vaccines, cell banks, and virus cultures.

Features

Ready-to-use convenience — no additional preparation steps are required.
Broad pharmaceutical applications — suitable for biopharmaceuticals, vaccines, cell cultures, and virus cultures.
Storage stability — can be stored at 2–8 °C for up to 3 months or at −20 °C for 6 months.
Rapid detection — a colorimetric chart provides a quick and convenient visual indication of early-stage mycoplasma proliferation.
Optimized growth performance — the buffered system supports higher colony counts while reducing the risk of mycoplasma cell death.
Enhanced visualization — a chromogenic reagent selectively stains mycoplasmas, improving clarity and specificity under microscopy.
Improved NAD⁺ stability — to prevent growth inhibition caused by NAD⁺ degradation during later stages, alternative NAD⁺ analogs are co-supplemented in the culture system.

Documents

M-FREY-Y SDS

Manual

Frey broth (M-FREY-Y)