{"product_id":"fluorometric-protein-quantification-kit","title":"Biofargo Fluorometric Protein Quantification Kit","description":"\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eDescription\u003c\/h2\u003e\n\u003cp\u003eBiofargo Fluorometric Protein Quantification Kit measures protein concentration in solution using a fluorogenic dye. The dye gives little signal on its own and becomes strongly fluorescent once bound to protein, so the fluorescence read after a short incubation is proportional to the amount of protein in the sample.\u003c\/p\u003e\n\u003cp\u003eFluorescence detection reaches lower protein concentrations than absorbance-based methods such as BCA and Bradford, so a smaller volume of a precious sample is used up per measurement. The assay is run as a mix-and-read procedure: sample and working reagent are combined, incubated 15 minutes at room temperature, and read directly, with no separation or wash steps.\u003c\/p\u003e\n\u003cp\u003eThe kit is supplied in two sizes. The 100-reaction size (BF52041001) suits method set-up and small sample sets; the 1,000-reaction size (BF52041002) suits routine and higher-throughput quantification. Both sizes use the same reagent formulation, so results are comparable between them.\u003c\/p\u003e\n\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable border=\"1\" style=\"border-collapse: collapse; width: 90%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eParameter\u003c\/strong\u003e\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e\u003cstrong\u003eSpecification\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProduct Name\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFluorometric Protein Quantification Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eCatalog Numbers\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBF52041001 (100 reactions) \/ BF52041002 (1,000 reactions)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAssay Principle\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFluorogenic dye binding; fluorescence proportional to protein concentration\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eDetection\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFluorescence\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eExcitation \/ Emission\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e470 \/ 570 nm (read with 485 \/ 590 nm filters)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eAssay Range\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e0.25–5 µg protein per reaction; pseudolinear response 0.5–4 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSample Concentration Range\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e12.5 µg\/mL – 5 mg\/mL before dilution\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eSample Volume\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e1–20 µL per reaction; final assay volume 200 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eWorking Solution\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eReagent diluted 1:200 in the supplied buffer, prepared fresh\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIncubation\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e15 minutes at room temperature; signal stable for up to 3 hours\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eCompatible Instruments\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBiofargo Fluorometer X1 and X3 (Protein program); Qubit™ fluorometers running the Protein assay; fluorescence microplate readers with 485 \/ 590 nm filters\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eProtein Standard\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eBovine serum albumin (BSA) standards included for the calibration curve\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eInterference Tolerance\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eTolerates 1 mM DTT and 1 mM β-mercaptoethanol. Detergents (SDS, Tween™ 20, Triton™ X-100) are not recommended\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003ePack Sizes\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003e100 reactions; 1,000 reactions\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eStorage\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eReagent at room temperature, desiccated and protected from light; buffer and standards at 2–8 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eShelf Life\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eAt least 6 months from receipt when stored as directed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd style=\"text-align: center;\"\u003eIntended Use\u003c\/td\u003e\n\u003ctd style=\"text-align: center;\"\u003eFor research use only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eFeatures\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eFluorescence detection\u003c\/strong\u003e reaches lower protein concentrations than colorimetric BCA or Bradford assays, so less sample is consumed per measurement.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eMix-and-read format\u003c\/strong\u003e — combine sample and working reagent, incubate 15 minutes, and read; no separation, wash or stop steps.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003e1–20 µL of sample\u003c\/strong\u003e per reaction, so dilute lysates and small eluate volumes can be measured directly.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eTwo pack sizes\u003c\/strong\u003e — 100 reactions for method set-up and small studies, 1,000 reactions for routine and higher-throughput work.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eSame formulation across both sizes\u003c\/strong\u003e, so a method developed on the small kit transfers directly to the large kit.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eRuns on \u003ca href=\"\/products\/fluorometer-x1-dna-rna-protein-quantification\"\u003eBiofargo Fluorometer X1\u003c\/a\u003e and \u003ca href=\"\/products\/fluorometer-x3-for-dna-rna-protein-quantification\"\u003eBiofargo Fluorometer X3\u003c\/a\u003e\u003c\/strong\u003e as well as on Qubit™ fluorometers and fluorescence microplate readers.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eStandard-curve quantification\u003c\/strong\u003e — samples are read against a calibration curve run alongside them, so each run carries its own calibration rather than a stored factor.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eSample normalization:\u003c\/strong\u003e Equalize protein loading across lysates before SDS-PAGE, Western blot or ELISA.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eProtein purification:\u003c\/strong\u003e Track protein in column fractions during chromatography and concentration steps.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003ePurified protein and antibody QC:\u003c\/strong\u003e Confirm the concentration of recombinant proteins and antibody preparations.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eEnzyme and reporter assays:\u003c\/strong\u003e Express activity per milligram of total protein.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eProteomics sample prep:\u003c\/strong\u003e Check protein input before digestion and mass spectrometry.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003e\u003cstrong\u003eLow-abundance samples:\u003c\/strong\u003e Quantify dilute lysates, eluates and conditioned media where colorimetric assays lack sensitivity.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eStorage \u0026amp; Shipping\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\u003cp\u003eShip at ambient temperature.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eStore the fluorescent reagent at room temperature, desiccated and protected from light.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eStore the assay buffer and the BSA standards at 2–8 °C.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eShelf life is at least 6 months from receipt when stored as directed.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eProtect the fluorogenic reagent from light; keep vials closed when not in use.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eProtocol Overview\u003c\/h2\u003e\n\u003col\u003e\n\u003cli\u003e\u003cp\u003ePrepare working solution by diluting the fluorescent reagent 1:200 in the supplied assay buffer. Prepare enough for all samples and standards, and prepare it fresh on the day of use.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eSet up the standards: add 10 µL of each BSA standard to 190 µL of working solution.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eSet up the samples: add 1–20 µL of sample and bring the volume to 200 µL with working solution. Choose the input volume so the protein mass falls within 0.25–5 µg.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eMix by vortexing 2–3 seconds, avoiding bubbles.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eIncubate 15 minutes at room temperature, protected from light.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eRead on the Protein program of the fluorometer, or at 485 \/ 590 nm on a microplate reader. Read standards first to build the calibration curve, then read the samples.\u003c\/p\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cp\u003eCalculate the original sample concentration from the reading and the sample volume used. Dilute and re-measure any sample that reads above the top standard.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003cstyle\u003e\n  .bf-rich .josh-faq { background: #fff; color: #000; font-family: \"IBM Plex Sans\", sans-serif; }\n  .bf-rich .josh-faq__inner { padding: 24px 0 40px; max-width: 100%; box-sizing: border-box; }\n  .bf-rich .josh-faq__item { border-bottom: 1px solid #e5e7eb; padding: 0; }\n  .bf-rich .josh-faq__item:first-child { border-top: 1px solid #e5e7eb; }\n  .bf-rich .josh-faq__question { width: 100%; margin: 0; padding: 20px 0; display: flex; align-items: center; justify-content: space-between; gap: 16px; color: #505052; background: none; border: 0; font: 700 16px\/1.4 \"IBM Plex Sans\", sans-serif; text-align: left; cursor: pointer; list-style: none; }\n  .bf-rich .josh-faq__question::-webkit-details-marker { display: none; }\n  .bf-rich .josh-faq__question:hover { color: #505052; }\n  .bf-rich .josh-faq__icon { position: relative; width: 20px; height: 20px; flex: 0 0 20px; transition: transform .25s ease; }\n  .bf-rich .josh-faq__icon::before, .bf-rich .josh-faq__icon::after { position: absolute; content: \"\"; background: currentColor; border-radius: 2px; transition: opacity .25s ease, transform .25s ease; }\n  .bf-rich .josh-faq__icon::before { top: 50%; left: 0; width: 100%; height: 2px; transform: translateY(-50%); }\n  .bf-rich .josh-faq__icon::after { top: 0; left: 50%; width: 2px; height: 100%; transform: translateX(-50%); }\n  .bf-rich .josh-faq__item[open] .josh-faq__icon::after { opacity: 0; transform: translateX(-50%) rotate(90deg); }\n  .bf-rich .josh-faq__answer { max-width: 860px; padding: 0 0 20px; color: #505052; font: 400 16px\/1.6 \"IBM Plex Sans\", sans-serif; }\n  .bf-rich .josh-faq__answer p { margin: 0 0 15px; color: #505052; font: inherit; }\n  .bf-rich .josh-faq__answer p:last-child { margin-bottom: 0; }\n  .bf-rich .josh-faq__answer ul { margin: 8px 0 12px; padding-left: 22px; }\n  .bf-rich .josh-faq__answer li { margin-bottom: 6px; font: inherit; list-style-type: disc; }\n  @media (max-width: 767px) { .bf-rich .josh-faq__inner { padding: 24px 0 40px; } .bf-rich .josh-faq__question { padding: 16px 0; font-size: 15px; } .bf-rich .josh-faq__answer { font-size: 15px; } }\n\u003c\/style\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eFrequently Asked Questions (FAQs)\u003c\/h2\u003e\n\u003cp\u003eCommon questions about Fluorometric Protein Quantification Kit.\u003c\/p\u003e\n\u003cdiv class=\"josh-faq\"\u003e\u003csection class=\"josh-faq__inner\"\u003e\u003cdiv class=\"josh-faq__list\"\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eHow is this different from a BCA or Bradford assay?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eBCA and Bradford assays measure absorbance, while this kit measures fluorescence. Fluorescence detection reaches lower protein concentrations, so smaller sample volumes can be used. BCA and Bradford remain useful where a plate reader without fluorescence optics is the only instrument available.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eWhat instrument do I need to run the assay?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eThe assay runs on \u003ca href=\"\/products\/fluorometer-x1-dna-rna-protein-quantification\"\u003eBiofargo Fluorometer X1\u003c\/a\u003e and \u003ca href=\"\/products\/fluorometer-x3-for-dna-rna-protein-quantification\"\u003eBiofargo Fluorometer X3\u003c\/a\u003e using the Protein program, on Qubit™ fluorometers running the Protein assay, and on fluorescence microplate readers fitted with 485 \/ 590 nm filters. Compatibility with Qubit™ instruments does not imply Thermo Fisher endorsement or identical performance. If you are unsure about your reader, contact us at contact@biofargo.com and we will confirm before you order.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eWhat is the assay range?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eEach reaction measures 0.25–5 µg of protein, with a pseudolinear response between 0.5 and 4 µg. That corresponds to an initial sample concentration of roughly 12.5 µg\/mL to 5 mg\/mL, depending on how much sample you add. Protein mass equals the initial concentration multiplied by the sample volume; dilute samples that read above the top standard.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eHow much sample is required?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eAdd 1–20 µL of sample and bring the reaction to a final volume of 200 µL with working solution. Choose the input volume so the protein mass lies within the assay range.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eWhich pack size should I choose?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eThe 100-reaction kit suits method set-up, occasional use and small sample sets. The 1,000-reaction kit lowers the cost per reaction and suits routine quantification or screening. Both use the same reagent formulation.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eIs a protein standard included?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eYes. Bovine serum albumin (BSA) standards are supplied for the calibration curve. Run the standards alongside your samples in every batch rather than relying on a stored curve.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eDoes the assay tolerate detergents and reducing agents in my buffer?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eReducing agents are tolerated at 1 mM DTT and 1 mM β-mercaptoethanol. Detergents such as SDS, Tween™ 20 and Triton™ X-100 are not recommended and will bias the reading. If your lysis buffer contains detergent, dilute the sample so the detergent is well below its working concentration, or tell us which buffer you use at contact@biofargo.com and we will confirm whether the assay is compatible.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eCan I use it with a 96-well plate?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eYes. The 200 µL assay volume suits a 96-well microplate on a fluorescence reader with 485 \/ 590 nm filters. Use black plates to limit well-to-well crosstalk and background.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eDo you offer bulk or distributor pricing?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eYes. Bulk and distributor pricing is quoted on request. Contact us at contact@biofargo.com with the quantity you need.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003cdetails class=\"josh-faq__item\"\u003e\u003csummary class=\"josh-faq__question\"\u003eCan it be used for clinical or diagnostic purposes?\u003cspan class=\"josh-faq__icon\" aria-hidden=\"true\"\u003e\u003c\/span\u003e\u003c\/summary\u003e\u003cdiv class=\"josh-faq__answer\"\u003e\u003cp\u003eNo. This product is for research use only and is not intended for human, diagnostic or therapeutic use.\u003c\/p\u003e\u003c\/div\u003e\u003c\/details\u003e\n\u003c\/div\u003e\u003c\/section\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cscript type=\"application\/ld+json\"\u003e{\"@context\": \"https:\/\/schema.org\", \"@type\": \"FAQPage\", \"mainEntity\": [{\"@type\": \"Question\", \"name\": \"How is this different from a BCA or Bradford assay?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"BCA and Bradford assays measure absorbance, while this kit measures fluorescence. Fluorescence detection reaches lower protein concentrations, so smaller sample volumes can be used. BCA and Bradford remain useful where a plate reader without fluorescence optics is the only instrument available.\"}}, {\"@type\": \"Question\", \"name\": \"What instrument do I need to run the assay?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"The assay runs on Biofargo Fluorometer X1 and Biofargo Fluorometer X3 using the Protein program, on Qubit\u0026trade; fluorometers running the Protein assay, and on fluorescence microplate readers fitted with 485 \/ 590 nm filters. Compatibility with Qubit\u0026trade; instruments does not imply Thermo Fisher endorsement or identical performance. If you are unsure about your reader, contact us at contact@biofargo.com and we will confirm before you order.\"}}, {\"@type\": \"Question\", \"name\": \"What is the assay range?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Each reaction measures 0.25\u0026ndash;5 \u0026#181;g of protein, with a pseudolinear response between 0.5 and 4 \u0026#181;g. That corresponds to an initial sample concentration of roughly 12.5 \u0026#181;g\/mL to 5 mg\/mL, depending on how much sample you add. Protein mass equals the initial concentration multiplied by the sample volume; dilute samples that read above the top standard.\"}}, {\"@type\": \"Question\", \"name\": \"How much sample is required?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Add 1\u0026ndash;20 \u0026#181;L of sample and bring the reaction to a final volume of 200 \u0026#181;L with working solution. Choose the input volume so the protein mass lies within the assay range.\"}}, {\"@type\": \"Question\", \"name\": \"Which pack size should I choose?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"The 100-reaction kit suits method set-up, occasional use and small sample sets. The 1,000-reaction kit lowers the cost per reaction and suits routine quantification or screening. Both use the same reagent formulation.\"}}, {\"@type\": \"Question\", \"name\": \"Is a protein standard included?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Yes. Bovine serum albumin (BSA) standards are supplied for the calibration curve. Run the standards alongside your samples in every batch rather than relying on a stored curve.\"}}, {\"@type\": \"Question\", \"name\": \"Does the assay tolerate detergents and reducing agents in my buffer?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Reducing agents are tolerated at 1 mM DTT and 1 mM \u0026beta;-mercaptoethanol. Detergents such as SDS, Tween\u0026trade; 20 and Triton\u0026trade; X-100 are not recommended and will bias the reading. If your lysis buffer contains detergent, dilute the sample so the detergent is well below its working concentration, or tell us which buffer you use at contact@biofargo.com and we will confirm whether the assay is compatible.\"}}, {\"@type\": \"Question\", \"name\": \"Can I use it with a 96-well plate?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Yes. The 200 \u0026#181;L assay volume suits a 96-well microplate on a fluorescence reader with 485 \/ 590 nm filters. Use black plates to limit well-to-well crosstalk and background.\"}}, {\"@type\": \"Question\", \"name\": \"Do you offer bulk or distributor pricing?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"Yes. Bulk and distributor pricing is quoted on request. Contact us at contact@biofargo.com with the quantity you need.\"}}, {\"@type\": \"Question\", \"name\": \"Can it be used for clinical or diagnostic purposes?\", \"acceptedAnswer\": {\"@type\": \"Answer\", \"text\": \"No. This product is for research use only and is not intended for human, diagnostic or therapeutic use.\"}}]}\u003c\/script\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eCompatible Instruments\u003c\/h2\u003e\n\u003cp\u003eThis kit is run on the Biofargo fluorometers below.\u003c\/p\u003e\n\u003cul\u003e\n  \u003cli\u003e\u003cp\u003e\u003ca href=\"\/products\/fluorometer-x1-dna-rna-protein-quantification\"\u003e\u003cstrong\u003eBiofargo Fluorometer X1\u003c\/strong\u003e\u003c\/a\u003e — Single sample position. Select the Protein program on the instrument.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp\u003e\u003ca href=\"\/products\/fluorometer-x3-for-dna-rna-protein-quantification\"\u003e\u003cstrong\u003eBiofargo Fluorometer X3\u003c\/strong\u003e\u003c\/a\u003e — Eight sample positions. Select the Protein program on the instrument.\u003c\/p\u003e\u003c\/li\u003e\n  \u003cli\u003e\u003cp\u003e\u003ca href=\"\/products\/s96-96-well-fluorometer-for-dna-rna-and-protein-quantification\"\u003e\u003cstrong\u003eBiofargo Fluorometer S96\u003c\/strong\u003e\u003c\/a\u003e — 96-well plate, full plate in 55 seconds. Select the Protein program on the instrument.\u003c\/p\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"bf-rich\"\u003e\n\u003ch2\u003eDocuments\u003c\/h2\u003e\n\u003cp\u003e\u003ca target=\"_blank\" rel=\"noopener\" href=\"https:\/\/biofargo.com\/pages\/contact\"\u003e\u003cstrong\u003eRequest Datasheet\u003c\/strong\u003e\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n","brand":"Biofargo","offers":[{"title":"100 Reactions","offer_id":53430945022133,"sku":"BF52041001","price":117.0,"currency_code":"USD","in_stock":true},{"title":"1,000 Reactions","offer_id":53430945054901,"sku":"BF52041002","price":473.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/biofargo.com\/products\/fluorometric-protein-quantification-kit","provider":"Biofargo","version":"1.0","type":"link"}