Description

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525nm.

It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins.
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer.

Specification

Grade	Conjugation
Formula	C21H11NO5S
MW	389.39
CAS	3326-32-7
Appearance	yellow-orange crystalline powder
Solution (1 % in ethanol/toluene 1:1)	yellow-orange, clear
Turbidity (1 % in ethanol/toluene 1:1)	≤2.0 NTU
Loss on Drying (3 h, 105 °C, vacuum)	≤5.0 %
purity(HPLC)	≤90 area-%
Thin Layer Chromatogram (50 mg in 5 ml of acetone; toluene/ethanol 1:1; UV 254 nm)	corresponds to reference material
Sulphated Ash	≤1.0 %
FTIR	corresponds to reference spectrum
Storage	4℃

Application

Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC)

Reagent for the FITC labeling of proteins; microsequencing of proteins and peptides (HPLC)

Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens. It may be employed as the derivatization reagent for amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine and P-phenylethylamine in human urine during their capillary electrophoretic (CE) determination. It may be used for the preparation of fluorescent antibodies. It was employed for in vitro sensitization studies.

Documents

COA

SDS

Disclaimer: For laboratory research use only.

Fluorescein isothiocyanate, Isomer I [FITC]